Pandit Raksha, Gaikwad Swapnil, Rai Mahendra
Department of Biotechnology, SGB Amravati University, Amravati 444 602, Maharashtra, India.
Microbial Diversity Research Center, Dr. D.Y. Patil Biotechnology and Bioinformatics Institute, Tathawade, Pune 411 033, Maharashtra, India.
IET Nanobiotechnol. 2017 Aug;11(5):568-575. doi: 10.1049/iet-nbt.2016.0165.
In the present study, the authors synthesised copper nanoparticles (CuNPs) by using extract of (ginger) and later the NPs were bioconjugated with nisin, which shows antimicrobial activity against food spoilage microorganisms. CuNPs and its bioconjugate were characterised by ultraviolet-vis spectroscopy, NP tracking analysis, Zetasizer, transmission electron microscopy analysis, X-ray diffraction and Fourier transform infra-red (FTIR) spectroscopy. Zeta potential of CuNPs and its bioconjugate were found to be very stable. They evaluated efficacy of CuNPs and its bioconjugate against selected food spoilage bacteria: namely, , and fungi including and Antimicrobial activity of CuNPs was found to be maximum against (18 mm) and the least activity was noted against (13 mm). Antioxidant activity of CuNPs and ginger extract was performed by various methods such as total antioxidant capacity reducing power assay, 1-1-diphenyl-2-picryl-hydrazyl free radical scavenging assay and hydrogen peroxide assay. Antioxidant activity of CuNPs was higher as compared with ginger extract. Hence, CuNPs and its bioconjugate can be used against food spoilage microorganisms.
在本研究中，作者使用（姜）提取物合成了铜纳米颗粒（CuNPs），随后将这些纳米颗粒与乳链菌肽进行生物共轭，乳链菌肽对食品腐败微生物具有抗菌活性。通过紫外可见光谱、纳米颗粒跟踪分析、Zetasizer、透射电子显微镜分析、X射线衍射和傅里叶变换红外（FTIR）光谱对CuNPs及其生物共轭物进行了表征。发现CuNPs及其生物共轭物的zeta电位非常稳定。他们评估了CuNPs及其生物共轭物对选定的食品腐败细菌（即 、 和真菌，包括 和 ）的功效。发现CuNPs对 的抗菌活性最高（直径18 mm），对 的活性最低（直径13 mm）。通过多种方法，如总抗氧化能力还原力测定、1,1-二苯基-2-苦基肼自由基清除测定和过氧化氢测定，对CuNPs和姜提取物的抗氧化活性进行了检测。与姜提取物相比，CuNPs的抗氧化活性更高。因此，CuNPs及其生物共轭物可用于对抗食品腐败微生物。
