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高通量转录组测序揭示高温胁迫下秋海棠属植物花色素苷代谢的作用。

High-throughput Transcriptome Sequencing Reveals the Role of Anthocyanin Metabolism in Begonia semperflorens Under High Light Stress.

机构信息

College of Forestry, Henan Agricultural University, Zhengzhou, Henan, China.

出版信息

Photochem Photobiol. 2018 Jan;94(1):105-114. doi: 10.1111/php.12813. Epub 2017 Sep 15.

DOI:10.1111/php.12813
PMID:28746749
Abstract

Begonia semperflorens is an ornamental perennial herb. The leaves of B. semperflorens turn red under increased light, which increases the ornamental value of the plant. The color of the leaves is determined by anthocyanin metabolism. In B. semperflorens leaves, anthocyanin metabolism is sensitive to external environmental conditions such as temperature, light and hormone levels. To explore this process in detail and to assess gene expression under high light stress, transcriptome analysis was performed by RNA sequencing using the sequencing-by-synthesis method. A total of 83 699 unigenes were isolated, and 51 754 unigenes were annotated using the NR, Swiss-Prot, KEGG, COG, KOG, GO and Pfam databases. Furthermore, many of the differentially expressed genes were related to factors associated with anthocyanin metabolism, which influences the expression of leaf color.

摘要

秋海棠是一种观赏多年生草本植物。在光照增强的情况下,秋海棠的叶子会变红,这增加了植物的观赏价值。叶子的颜色是由花青素代谢决定的。在秋海棠的叶子中,花青素代谢对外界环境条件(如温度、光照和激素水平)非常敏感。为了详细探索这一过程,并评估高光胁迫下的基因表达,我们使用基于合成测序法的 RNA 测序进行了转录组分析。共分离出 83699 个基因,使用 NR、Swiss-Prot、KEGG、COG、KOG、GO 和 Pfam 数据库注释了 51754 个基因。此外,许多差异表达基因与影响叶片颜色的花青素代谢相关因素有关。

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