锁核酸（LNA）增强了三唑连接的DNA与RNA的结合亲和力。
Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA.
作者信息
Kumar Pawan, El-Sagheer Afaf H, Truong Lynda, Brown Tom
机构信息
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
出版信息
Chem Commun (Camb). 2017 Aug 3;53(63):8910-8913. doi: 10.1039/c7cc05159j.
Oligonucleotides containing internal triazole-3'-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5'-side of the triazole does not stabilise duplexes. Triazole-LNA confers great resistance towards enzymatic degradation relative to LNA alone.
含有内部三唑-3'-锁核酸（LNA）连接的寡核苷酸与互补RNA结合时，其亲和力和特异性与未修饰的寡核苷酸相似，且明显优于仅含三唑的寡核苷酸。相比之下，三唑5'-侧的锁核酸（LNA）并不能使双链体稳定。相对于单独的锁核酸（LNA），三唑-锁核酸（LNA）对酶促降解具有很强的抗性。
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