平阳霉素预处理对眼部静脉畸形生物学行为的影响及其与半乳糖凝集素-3表达的关系。

Pingyangmycin Pretreatment Influences the Biological Behavior of Ocular Venous Malformation and Relates with Galectin-3 Expression.

作者信息

Li Jin, Qiao Jun-Bo, Liu Qiu-Yu

机构信息

Department of Ophthalmology, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Henan Eye Institute and Henan Eye Hospital, Zhengzhou, Henan 450052, China.

Department of Hemangioma Surgery, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan 450052, China.

出版信息

Chin Med J (Engl). 2017 Aug 5;130(15):1804-1809. doi: 10.4103/0366-6999.211537.

DOI:10.4103/0366-6999.211537

PMID:28748853

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5547832/

Abstract

BACKGROUND

Galectin-3 (Gal-3) plays a role in the mechanisms underlying ocular venous malformation. We conducted this study to investigate the effect of pingyangmycin pretreatment on the Gal-3 expressions and biological behavior of ocular venous malformation.

METHODS

Tissue samples were collected from 136 patients with ocular venous malformation. Patients were randomly divided into pingyangmycin (n = 69) and nonpingyangmycin group (n = 67). Patients in the pingyangmycin group received a local injection of 0.02% pingyangmycin once every 2 days for 2 weeks (7 doses) before removal surgery, whereas patients in the nonpingyangmycin group underwent removal surgery without local injection. The protein and messenger RNA (mRNA) expression of Gal-3 were detected by using immunohistochemistry and in situ hybridization.

RESULTS

Gal-3 protein was expressed in 35 (52%) of 67 samples in the nonpingyangmycin group and in 19 (28%) of 69 samples in the pingyangmycin group (P < 0.05). Gal-3 mRNA expression was detected in 39 (58%) of 67 samples in the nonpingyangmycin group and 22 (32%) of 69 samples in the pingyangmycin group (P < 0.05). The higher Gal-3 expressions were detected in samples with deeper invasiveness than those with superficial invasiveness before (χ2 = 12.720 and 13.369, respectively, both P < 0.05) and after pingyangmycin treatment (χ2 = 8.429 and 4.590, respectively, both P < 0.05). It was more frequently detected in mesh-like lesions with unclear boundary than round lesions with clear boundary before (χ2 = 30.291 and 41.466, respectively, both P < 0.05) and after pingyangmycin treatment (χ2 = 14.619 and 15.130, respectively, both P < 0.05). Pingyangmycin treatment led to a significant difference in Gal-3 expressions at both protein and mRNA levels (χ2 = 8.664 and 9.524, respectively, both P < 0.05).

CONCLUSIONS

Gal-3 expression may be involved in the development and invasiveness of ocular venous malformation, and pingyangmycin can inhibit Gal-3 expression, indicating a role of pingyangmycin treatment before the removal of ocular venous malformation.

摘要

背景

半乳糖凝集素-3（Gal-3）在眼部静脉畸形的发病机制中起作用。我们开展本研究以探讨平阳霉素预处理对眼部静脉畸形中Gal-3表达及生物学行为的影响。

方法

收集136例眼部静脉畸形患者的组织样本。患者被随机分为平阳霉素组（n = 69）和非平阳霉素组（n = 67）。平阳霉素组患者在切除手术前每2天局部注射1次0.02%平阳霉素，共2周（7剂），而非平阳霉素组患者未进行局部注射直接接受切除手术。采用免疫组织化学和原位杂交检测Gal-3的蛋白和信使核糖核酸（mRNA）表达。

结果

非平阳霉素组67个样本中有35个（52%）表达Gal-3蛋白，平阳霉素组69个样本中有19个（28%）表达Gal-3蛋白（P < 0.05）。非平阳霉素组67个样本中有39个（58%）检测到Gal-3 mRNA表达，平阳霉素组69个样本中有22个（32%）检测到Gal-3 mRNA表达（P < 0.05）。在平阳霉素治疗前及治疗后，浸润较深的样本中Gal-3表达均高于浸润较浅的样本（χ2分别为12.720和13.369，均P < 0.05；χ2分别为8.429和4.590，均P < 0.05）。在平阳霉素治疗前及治疗后，边界不清的网状病变中Gal-3表达均比边界清晰的圆形病变更常见（χ2分别为30.291和41.466，均P < 0.05；χ2分别为14.619和15.130，均P < 0.05）。平阳霉素治疗导致Gal-3在蛋白和mRNA水平的表达均有显著差异（χ2分别为8.664和9.524，均P < 0.05）。