Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Dis Model Mech. 2017 Sep 1;10(9):1155-1164. doi: 10.1242/dmm.028811. Epub 2017 Jul 28.
The zebrafish is a prominent vertebrate model for low-cost whole organism screening. In our recent screening of the distribution patterns of fluorescent compounds in live zebrafish larvae, fifteen compounds with tissue-specific distributions were identified. Several compounds were observed to accumulate in tissues where they were reported to induce side-effects, and compounds with similar structures tended to be enriched in the same tissues, with minor differences. In particular, we found three novel red fluorescent bone-staining dyes: purpurin, lucidin and 3-hydroxy-morindone; purpurin can effectively label bones in both larval and adult zebrafish, as well as in postnatal mice without significantly affecting bone mass and density. Moreover, two structurally similar chemotherapeutic compounds, doxorubicin and epirubicin, were observed to have distinct distribution preferences in zebrafish. Epirubicin maintained a relatively higher concentration in the liver, and performed better in inhibiting hepatic hyperplasia caused by the over-expression of In total, our study suggests that the transparent zebrafish larvae serve as valuable tools for identifying tissue-specific distributions of fluorescent compounds.
斑马鱼是一种用于低成本全器官筛选的重要脊椎动物模型。在我们最近对活体斑马鱼幼虫中荧光化合物分布模式的筛选中，鉴定出了 15 种具有组织特异性分布的化合物。一些化合物被观察到在其被报道引起副作用的组织中积累，并且具有相似结构的化合物往往在同一组织中富集，只有微小的差异。特别是，我们发现了三种新型红色荧光骨染色染料：紫红素、卢西定和 3-羟基马钱子碱；紫红素可以有效地标记幼鱼和成年斑马鱼以及产后小鼠的骨骼，而不会显著影响骨量和密度。此外，两种结构相似的化疗药物阿霉素和表阿霉素在斑马鱼中表现出不同的分布偏好。表阿霉素在肝脏中保持相对较高的浓度，并且在抑制由过度表达引起的肝增生方面表现更好。总的来说，我们的研究表明透明的斑马鱼幼虫是识别荧光化合物组织特异性分布的有价值工具。
