Khan Anum Khalid, Khan Khalid Mohammed, Ahmed Ayaz, Taha Muhammad, Perveen Shahnaz
H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
Microb Pathog. 2017 Sep;110:497-506. doi: 10.1016/j.micpath.2017.07.040. Epub 2017 Jul 26.
Antibacterial/antibiofilm potential of microwave-assisted synthetic thirty-three 2-amino-5-chloro benzophenone Schiff bases have been carried out against four bacterial strains i.e. Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus and Streptococcus mutans. Among them compounds 5, 6, 8, 9, 14, 16, 22, 24, 26, and 30-32 showed antibiofilm activities against isolates at less than 100 μg/ml concentrations. These compounds showed enhanced antibiofilm activity against S. aureus as compared to cefixime used as control. However, remaining compounds were found to be active but at higher concentration. Fluorescence microscopy has been employed for confirmation of antibiofilm results. The structures of all synthetic molecules have been characterized on the basis of spectroscopic techniques including H NMR, C NMR, EI-MS, HREI-MS, and IR spectroscopy and their structure-activity relationship have been established.
已对微波辅助合成的33种2-氨基-5-氯二苯甲酮席夫碱针对四种细菌菌株，即肺炎克雷伯菌、奇异变形杆菌、金黄色葡萄球菌和变形链球菌的抗菌/抗生物膜潜力进行了研究。其中，化合物5、6、8、9、14、16、22、24、26以及30 - 32在浓度低于100μg/ml时对分离株表现出抗生物膜活性。与用作对照的头孢克肟相比，这些化合物对金黄色葡萄球菌表现出增强的抗生物膜活性。然而，其余化合物被发现具有活性，但浓度较高。已采用荧光显微镜来确认抗生物膜结果。所有合成分子的结构已通过包括1H NMR、13C NMR、EI-MS、HREI-MS和红外光谱在内的光谱技术进行了表征，并建立了它们的构效关系。
