Tang Hai-Bo, Chen Fenglian, Rao Guibo, Bai Anbin, Jiang Jiajia, Du Yichao, Ren Pengfei, Liu Jinfeng, Qin Shaomin, Yang Lei, Wu Jianmin
Guangxi Veterinary Research Institute, Nanning, 530001, China.
Guangxi Veterinary Research Institute, Nanning, 530001, China.
Vet Microbiol. 2017 Aug;207:280-285. doi: 10.1016/j.vetmic.2017.06.018. Epub 2017 Jul 6.
To identify the causative agents in 3 large-scale outbreaks of encephalitis and death among farmed bamboo rats (Rhizomys pruinosus). The routine bacterial culture and identification were performed. There were no significant pathogenic bacteria isolated from the brain, heart, liver, spleen, lung, or kidney of diseased bamboo rats. Using PCR-based methods, we excluded the following as causative agent: pox virus, herpesvirus, adenovirus, lymphocytic choriomeningitis virus, rabies virus, and sendai virus. Furthermore, the homogenate from the diseased bamboo rats was subjected to viral metagenomic analysis which revealed 48506 filtered viral reads annotated to Akabane virus (AKAV) with >75% nucleotide identity, suggesting the presence of AKAVs in bamboo rats. Five novel AKAV isolates were successfully isolated and characterized. Furthermore the newly isolated AKAV isolate was used to demonstrate that it can reproduce the severe encephalitic and pneumonic disease in bamboo rats and mice. The findings add to the better understanding of AKAV epidemiology and to the prevention and control of Akabane diseases in China.
为确定3起养殖竹鼠（中华竹鼠）大规模脑炎及死亡疫情的病原体，进行了常规细菌培养与鉴定。在患病竹鼠的脑、心、肝、脾、肺或肾中未分离到显著致病细菌。采用基于聚合酶链反应（PCR）的方法，排除了痘病毒、疱疹病毒、腺病毒、淋巴细胞性脉络丛脑膜炎病毒、狂犬病病毒和仙台病毒作为病原体。此外，对患病竹鼠的匀浆进行病毒宏基因组分析，结果显示有48506条经过滤的病毒序列注释为赤羽病毒（AKAV），核苷酸同一性>75%，表明竹鼠中存在赤羽病毒。成功分离并鉴定了5株新型赤羽病毒毒株。此外，新分离的赤羽病毒毒株被用于证明其可在竹鼠和小鼠中引发严重的脑炎和肺炎疾病。这些发现有助于更好地了解赤羽病毒流行病学以及中国赤羽病的预防和控制。
