Gatti P J, Norman W P, Taveira Dasilva A M, Gillis R A
Brain Res. 1986 Sep 3;381(2):281-8. doi: 10.1016/0006-8993(86)90078-8.
The purpose of our study was to use microinjections of L-glutamic acid to better localize the cell bodies in the intermediate area of the ventral medullary surface that exert control over cardiorespiratory activity. L-glutamic acid (200 nl of a 1-M solution) was microinjected into the nucleus paragigantocellularis lateralis, lateral reticular nucleus and into an area which is part of the 'glycine-sensitive area', which lies in the center of the intermediate area. Normally, when L-glutamic acid is applied to the surface of the intermediate area, increases in arterial pressure and tidal volume are observed. Increases in tidal volume were never observed upon microinjection into the 3 sites associated with the intermediate area, suggesting that the tidal volume change elicited from surface application occurs because of L-glutamic acid interacting with cell bodies either on the surface or extremely close to the surface. Pressor responses were elicited with microinjection of L-glutamic acid into the lateral reticular nucleus and the 'glycine-sensitive area', but not the nucleus paragigantocellularis lateralis; indeed, microinjection of L-glutamic acid into the nucleus paragigantocellularis lateralis caused hypotension. Hence, cell bodies responsible for raising arterial pressure may reside in either the lateral reticular nucleus or the 'glycine-sensitive area'.
我们研究的目的是通过微量注射L-谷氨酸来更好地定位延髓腹侧面中间区域中对心肺活动施加控制的细胞体。将L-谷氨酸（1 M溶液200 nl）微量注射到外侧巨细胞旁核、外侧网状核以及位于中间区域中心的“甘氨酸敏感区”的一部分区域。通常，当将L-谷氨酸应用于中间区域表面时，会观察到动脉血压和潮气量增加。向与中间区域相关的3个部位微量注射时，从未观察到潮气量增加，这表明从表面应用引发的潮气量变化是由于L-谷氨酸与表面或极其靠近表面的细胞体相互作用所致。将L-谷氨酸微量注射到外侧网状核和“甘氨酸敏感区”时会引发升压反应，但注射到外侧巨细胞旁核时则不会；实际上，将L-谷氨酸微量注射到外侧巨细胞旁核会导致低血压。因此，负责升高动脉血压的细胞体可能位于外侧网状核或“甘氨酸敏感区”。
