Danese Elisa, Lippi Giuseppe, Buonocore Ruggero, Benati Marco, Bovo Chiara, Bonaguri Chiara, Salvagno Gian Luca, Brocco Giorgio, Roggenbuck Dirk, Montagnana Martina
Section of Clinical Biochemistry, University of Verona, Verona, Italy.
Laboratory of Clinical Chemistry and Haematology, Academic Hospital of Parma, Parma, Italy.
Ann Transl Med. 2017 Jul;5(13):272. doi: 10.21037/atm.2017.04.35.
The use of mobile phones has been associated with an increased risk of developing certain type of cancer, especially in long term users. Therefore, this study was aimed to investigate the potential genotoxic effect of mobile phone radiofrequency exposure on human peripheral blood mononuclear cells
The study population consisted in 14 healthy volunteers. After collection of two whole blood samples, the former was placed in a plastic rack, 1 cm from the chassis of a commercial mobile phone (900 MHz carrier frequency), which was activated by a 30-min call. The second blood sample was instead maintained far from mobile phones or other RF sources. The influence of mobile phone RF on DNA integrity was assessed by analyzing γ-H2AX foci in lymphocytes using immunofluorescence staining kit on AKLIDES.
No measure of γ-H2AX foci was significantly influenced by mobile phone RF exposure, nor mobile phone exposure was associated with significant risk of genetic damages (odds ratio comprised between 0.27 and 1.00).
The results of this experimental study demonstrate that exposure of human lymphocytes to a conventional 900 MHz RF emitted by a commercial mobile phone for 30 min does not significantly impact DNA integrity.
使用手机与患某些类型癌症的风险增加有关，尤其是长期使用者。因此，本研究旨在调查手机射频辐射对人外周血单个核细胞的潜在遗传毒性作用。
研究对象为14名健康志愿者。采集两份全血样本后，将前者置于塑料架中，距离商用手机（900MHz载波频率）的底盘1厘米，该手机通过30分钟通话激活。另一份血样则远离手机或其他射频源保存。通过使用AKLIDES上的免疫荧光染色试剂盒分析淋巴细胞中的γ-H2AX焦点，评估手机射频对DNA完整性的影响。
γ-H2AX焦点的测量值均未受到手机射频辐射的显著影响，手机暴露也与遗传损伤的显著风险无关（优势比在0.27至1.00之间）。
本实验研究结果表明，人淋巴细胞暴露于商用手机发出的常规900MHz射频30分钟不会显著影响DNA完整性。
