Cho Geon-Yeong, Lee Jae-Chan, Whang Kyung-Sook
Department of Microbial and Nano Materials, College of Science and Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.
Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 35349, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2598-2603. doi: 10.1099/ijsem.0.001981. Epub 2017 Jul 31.
Three strains of a Gram-stain-negative, moderately halophilic bacterium, designated strain KHM44T, KHM29 and HHM4, were isolated from the sediment of a grey saltern located on Sinui island at Shinan, Korea. The isolates were aerobic, non-motile rods and grew at 15-50 °C (optimum, 37 °C), at pH 6.0-9.0 (optimum, pH 8.0) and at salinities of 3-25 % (w/v) NaCl (optimum, 10 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the major fatty acids were iso-C15 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH, iso-C17 : 1ω9c and anteiso-C15 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and glycolipid. The DNA G+C contents were 48.5-48.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains KHM44T, KHM29 and HHM4 belonged to the genus Aliifodinibius in the family Balneolaceae, with sequence similarities of 95.1-97.2 % to members of this genus. Levels of DNA-DNA relatedness between strain KHM44T and the type strains of the other species of the genus Aliifodinibius ranged from 35.4 to 48.0 %. On the basis of polyphasic analysis from this study, strains KHM44T, KHM29 and HHM4 are considered to represent a novel species of the genus Aliifodinibius, for which the name Aliifodinibius salicampi sp. nov. is proposed. The type strain is KHM44T (=KACC 19060T=NBRC 112531T).
从韩国新安实尾岛一个灰色盐场的沉积物中分离出三株革兰氏染色阴性、中度嗜盐的细菌，分别命名为菌株KHM44T、KHM29和HHM4。这些分离菌株为需氧、无运动性的杆菌，在15-50°C（最适温度37°C）、pH 6.0-9.0（最适pH 8.0）和盐度3-25%（w/v）NaCl（最适10% NaCl）的条件下生长。主要的类异戊二烯醌是甲基萘醌-7（MK-7），主要脂肪酸为异-C15:0、C16:1ω7c和/或异-C15:0 2-OH、异-C17:1ω9c和anteiso-C15:0。极性脂类为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和糖脂。DNA G+C含量为48.5-48.7 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株KHM44T、KHM29和HHM4属于Balneolaceae科的Aliifodinibius属，与该属成员的序列相似性为95.1-97.2%。菌株KHM44T与Aliifodinibius属其他物种的模式菌株之间的DNA-DNA相关性水平在35.4%至48.0%之间。基于本研究的多相分析，菌株KHM44T、KHM29和HHM4被认为代表Aliifodinibius属的一个新物种，为此提出名为Aliifodinibius salicampi sp. nov.的名称。模式菌株为KHM44T（=KACC 19060T=NBRC 112531T）。
