Tsuji Masayoshi, Kanda Hideyuki, Hayakawa Takehito, Mori Yayoi, Ito Teruna, Hidaka Tomoo, Kakamu Takeyasu, Kumagai Tomohiro, Osaki Yoneatsu, Kawazoe Miki, Sato Sei, Fukushima Tetsuhito
Department of Preventive Medicine & Public Health, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Japan.
Department of Hygiene & Preventive Medicine, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Japan.
Cancer Biomark. 2017 Jul 19;20(1):41-48. doi: 10.3233/CBM-170004.
Nicotine concentration in hair is a useful marker of tobacco exposure. Detection of nicotine in the hair of non-smokers indicates passive smoking. Accurate measurement of nicotine among active and passive smokers can help in smoking cessation programs or programs designed to prevent secondhand smoke exposure.
To establish, using high-performance liquid chromatography-ultraviolet detection (HPLC/UV), a hair nicotine cut-off value to distinguish active from passive smokers.
Hair samples were collected from randomly chosen Japanese men (n= 192) between 2009 and 2011. Nicotine and cotinine levels in hair were measured using HPLC/UV with column-switching. T-tests and chi-square tests were performed to compare active and passive smokers, while receiver operating characteristic curves were used to evaluate the effectiveness of the cut-off value.
There were 69 active smokers and 123 passive smokers. The nicotine and cotinine concentrations in hair were significantly higher in active than in passive smokers (p< 0.01). The area under the curve for nicotine was 0.92. A hair nicotine cut-off value of 5.68 ng/mg, with a sensitivity of 94.2% and specificity of 87.0%, was identified as the optimal cut-off value for separating active from passive smokers.
Nicotine and cotinine concentrations in hair clearly distinguished active from passive smokers.
头发中的尼古丁浓度是烟草暴露的一个有用标志物。在不吸烟者头发中检测到尼古丁表明存在被动吸烟。准确测量主动和被动吸烟者体内的尼古丁有助于戒烟项目或旨在预防二手烟暴露的项目。
使用高效液相色谱-紫外检测法（HPLC/UV）确定一个头发尼古丁临界值，以区分主动吸烟者和被动吸烟者。
2009年至2011年期间，从随机选取的日本男性（n = 192）中收集头发样本。使用带柱切换的HPLC/UV测量头发中的尼古丁和可替宁水平。进行t检验和卡方检验以比较主动吸烟者和被动吸烟者，同时使用受试者工作特征曲线评估临界值的有效性。
有69名主动吸烟者和123名被动吸烟者。主动吸烟者头发中的尼古丁和可替宁浓度显著高于被动吸烟者（p < 0.01）。尼古丁的曲线下面积为0.92。头发尼古丁临界值为5.68 ng/mg，灵敏度为94.2%，特异性为87.0%，被确定为区分主动吸烟者和被动吸烟者的最佳临界值。
头发中的尼古丁和可替宁浓度能清楚地区分主动吸烟者和被动吸烟者。
