Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.
Department of Nanotechnology and Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
J Alzheimers Dis. 2017;59(4):1187-1202. doi: 10.3233/JAD-160206.
It is well understood that patients with different diseases may have a variety of specific proteins (e.g., type, amount, and configuration) in their plasmas. When nanoparticles (NPs) are exposed to these plasmas, the resulting coronas may incorporate some of the disease-specific proteins. Using gold (Au) NPs with different surface properties and corona composition, we have developed a technology for the discrimination and detection of two neurodegenerative diseases, Alzheimer's disease (AD) and multiple sclerosis (MS). Applying a variety of techniques, including UV-visible spectra, colorimetric response analyses and liquid chromatography-tandem mass spectrometry, we found the corona-NP complexes, obtained from different human serums, had distinct protein composition, including some specific proteins that are known as AD and MS biomarkers. The colorimetric responses, analyzed by chemometrics and statistical methods, demonstrate promising capabilities of the technology to unambiguously identify and discriminate AD and MS. The developed colorimetric technology might enable a simple, inexpensive and rapid detection/discrimination of neurodegenerative diseases.
众所周知，患有不同疾病的患者的血浆中可能具有多种特定蛋白质（例如，类型、数量和构型）。当纳米颗粒（NPs）暴露于这些血浆中时，所形成的冠层可能会包含一些疾病特异性蛋白质。我们使用具有不同表面性质和冠层组成的金（Au）NPs，开发了一种用于区分和检测两种神经退行性疾病（阿尔茨海默病（AD）和多发性硬化症（MS）的技术。通过应用多种技术，包括紫外可见光谱，比色响应分析和液相色谱-串联质谱法，我们发现来自不同人血清的冠层-NP 复合物具有不同的蛋白质组成，包括一些已知的 AD 和 MS 生物标志物。通过化学计量学和统计方法分析的比色响应表明，该技术具有明确识别和区分 AD 和 MS 的有前途的能力。所开发的比色技术可能能够实现对神经退行性疾病的简单，廉价和快速检测/区分。
