血清淀粉样蛋白 A 富集损害了糖尿病肾病患者 HDL 的抗炎能力。

Serum amyloid A enrichment impairs the anti-inflammatory ability of HDL from diabetic nephropathy patients.

机构信息

Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

出版信息

J Diabetes Complications. 2017 Oct;31(10):1538-1543. doi: 10.1016/j.jdiacomp.2017.07.005. Epub 2017 Jul 13.

DOI:10.1016/j.jdiacomp.2017.07.005

PMID:28760652

Abstract

AIMS

Impaired anti-inflammatory ability of high-density lipoprotein (HDL) has been demonstrated in patients with type-2 diabetes mellitus (T2DM). However, whether HDL from patients with diabetic nephropathy (DN) suffers additional damage remains unknown. This study compared the anti-inflammatory capacities of HDL from healthy controls, T2DM patients with normal renal function, and T2DM patients with DN.

MATERIALS AND METHODS

HDL was isolated from healthy controls (n=33) and T2DM patients with normal renal function (n=21), chronic kidney disease (CKD) (n=27), and end-stage renal disease (ESRD) (n=27). Human peripheral blood mononuclear cells (PBMCs) from healthy volunteers were pretreated with HDL (100μg/mL) for 1h, then incubated with lipopolysaccharide (LPS) (50ng/mL) for 24h. The anti-inflammatory ability of HDL was measured as the secretion of TNF-α in LPS-activated monocytes.

RESULTS

The anti-inflammatory ability of HDL was gradually impaired as kidney function declined. Serum amyloid A (SAA) concentration in HDL significantly increased and was positively correlated with the impaired anti-inflammatory ability of HDL (Pearson r=0.315, P=0.006). Furthermore, HDL supplemented with SAA significantly increased TNF-α release from PBMCs compared with that from control HDL.

CONCLUSIONS

These findings identified an impaired anti-inflammatory capacity of HDL from DN patients, which might be attributable to SAA enrichment.

摘要

目的

已有研究表明，2 型糖尿病（T2DM）患者的高密度脂蛋白（HDL）抗炎能力受损。然而，糖尿病肾病（DN）患者的 HDL 是否受到进一步损伤尚不清楚。本研究比较了健康对照者、肾功能正常的 T2DM 患者和 DN 患者的 HDL 的抗炎能力。

材料和方法

从健康对照者（n=33）和肾功能正常的 T2DM 患者（n=21）、慢性肾脏病（CKD）（n=27）和终末期肾病（ESRD）（n=27）患者中分离 HDL。用 HDL（100μg/mL）预处理健康志愿者外周血单个核细胞（PBMCs）1h，然后用脂多糖（LPS）（50ng/mL）孵育 24h。用 LPS 激活单核细胞后，通过 TNF-α 的分泌来测量 HDL 的抗炎能力。