依赖因子的古菌转录终止。
Factor-dependent archaeal transcription termination.
机构信息
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523.
出版信息
Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):E6767-E6773. doi: 10.1073/pnas.1704028114. Epub 2017 Jul 31.
RNA polymerase activity is regulated by nascent RNA sequences, DNA template sequences, and conserved transcription factors. Transcription factors promoting initiation and elongation have been characterized in each domain, but transcription termination factors have been identified only in bacteria and eukarya. Here we describe euryarchaeal termination activity (Eta), the first archaeal termination factor capable of disrupting the transcription elongation complex (TEC), detail the rate of and requirements for Eta-mediated transcription termination, and describe a role for Eta in transcription termination in vivo. Eta-mediated transcription termination is energy-dependent, requires upstream DNA sequences, and disrupts TECs to release the nascent RNA to solution. Deletion of TK0566 (encoding Eta) is possible, but results in slow growth and renders cells sensitive to DNA damaging agents. Our results suggest that the mechanisms used by termination factors in archaea, eukarya, and bacteria to disrupt the TEC may be conserved, and that Eta stimulates release of stalled or arrested TECs.
RNA 聚合酶活性受新生 RNA 序列、DNA 模板序列和保守转录因子的调节。在每个领域都已经鉴定出促进起始和延伸的转录因子，但只有在细菌和真核生物中才鉴定出转录终止因子。在这里，我们描述了广古菌终止活性（Eta），这是第一个能够破坏转录延伸复合物（TEC）的古菌终止因子，详细描述了 Eta 介导的转录终止的速率和要求，并描述了 Eta 在体内转录终止中的作用。Eta 介导的转录终止是能量依赖性的，需要上游 DNA 序列，并破坏 TEC 以将新生 RNA 释放到溶液中。TK0566（编码 Eta）的缺失是可能的，但会导致生长缓慢并使细胞对 DNA 损伤剂敏感。我们的结果表明，终止因子在古菌、真核生物和细菌中用于破坏 TEC 的机制可能是保守的，并且 Eta 刺激停滞或停滞的 TEC 的释放。
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