Volkmann Elizabeth R, Hoffmann-Vold Anna-Maria, Chang Yu-Ling, Jacobs Jonathan P, Tillisch Kirsten, Mayer Emeran A, Clements Philip J, Hov Johannes R, Kummen Martin, Midtvedt Øyvind, Lagishetty Venu, Chang Lin, Labus Jennifer S, Molberg Øyvind, Braun Jonathan
Department of Medicine, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, California, USA.
Department of Rheumatology, Oslo University Hospital, Oslo, Norway.
BMJ Open Gastroenterol. 2017 Apr 1;4(1):e000134. doi: 10.1136/bmjgast-2017-000134. eCollection 2017.
To compare faecal microbial composition in patients with systemic sclerosis (SSc) from 2 independent cohorts with controls and to determine whether certain genera are associated with SSc-gastrointestinal tract (GIT) symptoms.
Adult patients with SSc from the University of California, Los Angeles (UCLA) and Oslo University Hospital (OUH) and healthy controls participated in this study (1:1:1). All participants provided stool specimens for 16S rRNA sequencing. Linear discriminant analysis effect size demonstrated genera with differential expression in SSc. Differential expression analysis for sequence count data identified specific genera associated with GIT symptoms as assessed by the GIT 2.0 questionnaire.
The UCLA-SSc and OUH-SSc cohorts were similar in age (52.1 and 60.5 years, respectively), disease duration (median (IQR): 6.6 (2.5-16.4) and 7.0 (1.0-19.2) years, respectively), gender distribution (88% and 71%, respectively), and GIT symptoms (mean (SD) total GIT 2.0 scores of 0.7 (0.6) and 0.6 (0.5), respectively). Principal coordinate analysis illustrated significant microbial community differences between SSc and controls (UCLA: p=0.001; OUH: p=0.002). Patients with SSc had significantly lower levels of commensal genera deemed to protect against inflammation, such as (UCLA and OUH), (UCLA), (OUH); and significantly higher levels of pathobiont genera, such as (UCLA), compared with controls. Increased abundance of was associated with less severe GIT symptoms in both cohorts.
The present analysis detected specific aberrations in the lower GIT microbiota of patients with SSc from 2 geographically and ethnically distinct cohorts. These findings suggest that GIT dysbiosis may be a pathological feature of the SSc disease state.
比较来自两个独立队列的系统性硬化症（SSc）患者与对照组的粪便微生物组成，并确定某些菌属是否与SSc胃肠道（GIT）症状相关。
来自加利福尼亚大学洛杉矶分校（UCLA）和奥斯陆大学医院（OUH）的成年SSc患者以及健康对照参与了本研究（1:1:1）。所有参与者均提供粪便标本用于16S rRNA测序。线性判别分析效应大小显示了SSc中差异表达的菌属。通过GIT 2.0问卷评估，对序列计数数据进行差异表达分析，确定了与GIT症状相关的特定菌属。
UCLA-SSc和OUH-SSc队列在年龄（分别为52.1岁和60.5岁）、病程（中位数（IQR）：分别为6.6（2.5 - 16.4）年和7.0（1.0 - 19.2）年）、性别分布（分别为88%和71%）以及GIT症状（GIT 2.0总评分的平均值（标准差）分别为0.7（0.6）和0.6（0.5））方面相似。主坐标分析表明SSc与对照组之间存在显著的微生物群落差异（UCLA：p = 0.001；OUH：p = 0.002）。与对照组相比，SSc患者中被认为具有抗炎作用的共生菌属水平显著降低，如（UCLA和OUH）、（UCLA）、（OUH）；而致病共生菌属水平显著升高，如（UCLA）。两个队列中，的丰度增加与较轻的GIT症状相关。
本分析在来自两个地理和种族不同队列的SSc患者的下消化道微生物群中检测到特定异常。这些发现表明GIT生态失调可能是SSc疾病状态的一个病理特征。
