Xu Yiwei, Zhang Wen, Shi Jiyong, Zou Xiaobo, Li Yanxiao, Haroon Elrasheid Tahir, Huang Xiaowei, Li Zhihua, Zhai Xiaodong, Hu Xuetao
Key Laboratory of Modern Agriculture Equipment and Technology, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Key Laboratory of Modern Agriculture Equipment and Technology, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2017 Dec 15;237:423-430. doi: 10.1016/j.foodchem.2017.05.096. Epub 2017 May 18.
Fish consumption is the main source of methylmercury (CHHg) exposure for humans. In this study, gold nanoparticles and reduced graphene oxide (AuNPs-RGO) modified electrode was fabricated for determination of CHHg in fish. The AuNPs-RGO composite was synthesized by electroreduction method. The composite was characterized by scanning electron microscopy and energy dispersive spectra, X-ray diffraction and Raman spectroscopy. Electrochemical performance of the proposed sensor was studied by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse stripping voltammetry. The excellent conductivity and large surface of graphene contributed to an improvement in the voltammetric stripping signal. Under the optimized conditions, the methylmercury concentration in the range from 3 to 24μgL had a good linear relation with the peak current. The detection limit of AuNPs-RGO modified electrode was 0.12μgL. Finally, the developed electrode was applied to detect methylmercury in fish samples, and the obtained results were in good agreement with certified values.
鱼类消费是人类接触甲基汞（CHHg）的主要来源。在本研究中，制备了金纳米颗粒和还原氧化石墨烯（AuNPs-RGO）修饰电极用于测定鱼类中的CHHg。采用电还原法合成了AuNPs-RGO复合材料。通过扫描电子显微镜和能谱、X射线衍射和拉曼光谱对复合材料进行了表征。采用循环伏安法、电化学阻抗谱和差分脉冲溶出伏安法研究了所提传感器的电化学性能。石墨烯优异的导电性和大表面积有助于提高伏安溶出信号。在优化条件下，甲基汞浓度在3至24μg/L范围内与峰电流具有良好的线性关系。AuNPs-RGO修饰电极的检测限为0.12μg/L。最后，将所制备的电极应用于检测鱼类样品中的甲基汞，所得结果与认证值吻合良好。
