da Silva Kelly Regina, Damasceno Jaqueline Lopes, Inácio Moana de Oliveira, Abrão Fariza, Ferreira Natália Helen, Tavares Denise Crispim, Ambrosio Sergio Ricardo, Veneziani Rodrigo Cassio Sola, Martins Carlos Henrique Gomes
Laboratory of Research in Applied Microbiology (LAPEMA), University of Franca, Franca, Brazil.
Institute of Biomedical Sciences (ICBIM), Federal University of Uberlândia, Uberlândia, Brazil.
Front Microbiol. 2019 May 7;10:987. doi: 10.3389/fmicb.2019.00987. eCollection 2019.
Considering the impact of dental caries on public health and the wide biological potential described for species belonging to the genus , here we investigate the antibacterial activity of the and resins and of the diterpene dehydroabietic acid (DHA) against cariogenic bacteria. For this purpose, we have determined the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the resins and of the diterpene. We have also investigated the biofilm inhibition ability (through MBIC determination), as well as the synergistic effect (through fractional inhibitory concentration index) and the cytotoxic potential (through XTT assay) of the and resins. The resins gave promising MIC and MBC values, which ranged from 12.5 to 400 μg/mL; DHA provided MIC and MBC values ranging from 25 to 400 μg/mL. The MICB values ranged from 0.78 to 400 μg/mL for the resins. Neither additive nor synergistic effects emerged for the combinations of one of the resins with chlorhexidine. The cytotoxic activity was ≥312.5 and ≥156.3 μg/mL for the and resins, respectively. The resins showed antibacterial activity against planktonic and sessile cariogenic bacteria. These data are relevant and encourage further research into these plants, which may contribute to the discovery of new substances that can inhibit the growth of cariogenic microorganisms and reduce the incidence of dental caries.
考虑到龋齿对公众健康的影响以及所述属物种具有的广泛生物学潜力，在此我们研究了[树脂名称1]和[树脂名称2]树脂以及二萜脱氢枞酸（DHA）对致龋菌的抗菌活性。为此，我们测定了树脂和二萜的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。我们还研究了[树脂名称1]和[树脂名称2]树脂的生物膜抑制能力（通过测定MBIC）以及协同效应（通过分数抑菌浓度指数）和细胞毒性潜力（通过XTT试验）。树脂给出了有前景的MIC和MBC值，范围为12.5至400μg/mL；DHA的MIC和MBC值范围为25至400μg/mL。树脂的MBIC值范围为0.78至400μg/mL。其中一种树脂与洗必泰的组合未出现相加或协同效应。[树脂名称1]和[树脂名称2]树脂的细胞毒性活性分别≥312.5和≥156.3μg/mL。这些树脂对浮游和固着的致龋菌均表现出抗菌活性。这些数据具有相关性，并鼓励对这些植物进行进一步研究，这可能有助于发现能够抑制致龋微生物生长并降低龋齿发病率的新物质。
