Hager K M, Mandala S M, Davenport J W, Speicher D W, Benz E J, Slayman C W
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7693-7. doi: 10.1073/pnas.83.20.7693.
The plasma membrane of Neurospora crassa contains an electrogenic H+-ATPase (EC 3.6.1.35), for which we have isolated and sequenced both genomic and cDNA clones. The ATPase gene is interrupted by four small introns (58-124 base pairs). It encodes a protein of 920 amino acids (Mr, 99,886) possessing as many as eight transmembrane segments. The Neurospora ATPase shows significant amino acid sequence homology with the Na+,K+- and Ca2+-transporting ATPases of animal cells, particularly in regions that appear to be involved in ATP binding and hydrolysis.