Hager K M, Mandala S M, Davenport J W, Speicher D W, Benz E J, Slayman C W
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7693-7. doi: 10.1073/pnas.83.20.7693.
The plasma membrane of Neurospora crassa contains an electrogenic H+-ATPase (EC 3.6.1.35), for which we have isolated and sequenced both genomic and cDNA clones. The ATPase gene is interrupted by four small introns (58-124 base pairs). It encodes a protein of 920 amino acids (Mr, 99,886) possessing as many as eight transmembrane segments. The Neurospora ATPase shows significant amino acid sequence homology with the Na+,K+- and Ca2+-transporting ATPases of animal cells, particularly in regions that appear to be involved in ATP binding and hydrolysis.
粗糙脉孢菌的质膜含有一种生电H⁺-ATP酶（EC 3.6.1.35），我们已分离出该酶的基因组克隆和cDNA克隆并进行了测序。ATP酶基因被四个小内含子（58 - 124个碱基对）中断。它编码一个含有多达八个跨膜区段的920个氨基酸的蛋白质（Mr，99,886）。粗糙脉孢菌的ATP酶与动物细胞的Na⁺,K⁺-和Ca²⁺-转运ATP酶显示出显著的氨基酸序列同源性，特别是在似乎参与ATP结合和水解的区域。
