Li Min, Yang Jianfeng, Li Haoyu, Wang Yating, Cheng Xu, Han Guodong, Bisseling Ton, Zhao Jun
Laboratory of Molecular Phytopathology, Horticultural and Plant Protection Department, Inner Mongolia Agricultural University, Hohhot, China.
The Modern Agricultural and Animal Husbandry Development Center of Bayannur, Bayannur, China.
Front Microbiol. 2024 Aug 27;15:1396044. doi: 10.3389/fmicb.2024.1396044. eCollection 2024.
Potato Verticillium wilt (PVW) caused by is a vascular disease, that seriously affects potato ( L.) yield and quality worldwide. occupies the vascular bundle and therefore it cannot efficiently be treated with fungicides. Further, the application of these pesticides causes serious environmental problems. Therefore, it is of great importance to find environmentally friendly biological control methods. In this study, bacterial strains were isolated from agricultural lands on which potato had been cultured for 5 years. Five strains with a broad-spectrum antagonistic activity were selected. Among these five strains, XS142 showed the highest antagonistic activity. To study the mechanism of XS142, by which this strain might confer tolerance to in potato, the genome of strain XS142 was sequenced. This showed that its genome has a high level of sequence identity with the model strain FZB42 as the OrthoANI (Average Nucleotide Identity by Orthology) value is 98%. The fungal suppressing mechanisms of this model strain are well studied. Based on the genome comparison it can be predicted that XS142 has the potential to suppress the growth of by production of bacillomycin D, fengycin, and chitinase. Further, the transcriptomes of potatoes treated with XS142 were analyzed and this showed that XS142 does not induce ISR, but the expression of genes encoding peptides with antifungal activity. Here we showed that XS142 is an endophyte. Further, it is isolated from a field where potato had been cultured for several years. These properties give it a high potential to be used, in the future, as a biocontrol agent of PVW in agriculture.
由[病原菌名称未给出]引起的马铃薯黄萎病（PVW）是一种维管束病害，在全球范围内严重影响马铃薯（[马铃薯学名未给出]）的产量和品质。[病原菌名称未给出]占据维管束，因此无法有效地用杀菌剂进行防治。此外，这些农药的使用会导致严重的环境问题。因此，寻找环境友好型生物防治方法至关重要。在本研究中，从种植马铃薯5年的农田中分离出细菌菌株。筛选出5株具有广谱拮抗活性的菌株。在这5株菌株中，[菌株名称未给出]XS142表现出最高的拮抗活性。为了研究XS142赋予马铃薯对[病原菌名称未给出]耐受性的机制，对菌株XS142的基因组进行了测序。结果表明，其基因组与模式菌株[模式菌株名称未给出]FZB42具有高度的序列同一性，直系同源平均核苷酸同一性（OrthoANI）值为98%。对该模式菌株的真菌抑制机制已有充分研究。基于基因组比较，可以预测XS142有可能通过产生杆菌霉素D、丰原素和几丁质酶来抑制[病原菌名称未给出]的生长。此外，对用XS142处理的马铃薯的转录组进行了分析，结果表明XS142不会诱导诱导性系统抗性（ISR），而是诱导编码具有抗真菌活性肽的基因表达。在这里我们表明XS142是一种内生菌。此外，它是从种植马铃薯数年的田间分离得到的。这些特性使其在未来具有作为农业中PVW生物防治剂的巨大潜力。
