血管紧张素转换酶基因（I/D）多态性与高血压及2型糖尿病的关联

Association of angiotensin converting enzyme gene (I/D) polymorphism with hypertension and type 2 diabetes.

作者信息

Zarouk W A, Hussein I R, Esmaeil N N, Raslan H M, Reheim H A A, Moguib O, Emara N A, Aly A A, Hamed M

机构信息

Molecular Genetic and Enzymology Department, National Research Centre, Cairo, Egypt.

出版信息

Bratisl Lek Listy. 2012;113(1):14-8. doi: 10.4149/bll_2012_003.

DOI:10.4149/bll_2012_003

PMID:22380495

Abstract

OBJECTIVES

This study was conducted to determine the association of insertion/deletion (I/D) polymorphism of the ACE gene in hypertensive and T2DM subjects in Egyptian population.

BACKGROUND

The deletion (D) allele of the angiotensin-converting enzyme (ACE) gene has been studied in relation to hypertension and type 2 diabetes mellitus (T2DM) with contradictory results which might be due to ethnic and geographical variations.

METHODS

A total of 85 subjects participated in this study; hypertension (Group 1); type 2 diabetes mellitus (Group 2) and controls (Group 3). Written informed consent was obtained. for each subject: age, sex, diabetes duration and the drugs used, blood pressure (systolic and diastolic), and lipid profile. Genotyping was performed by polymerase chain reaction (PCR).

RESULTS

The frequency of DD genotype was significantly higher in hypertensive (60 %) and diabetic patients (68 %) compared to controls (33.3 %) (p=0.04, p=0.01 respectively). The DD genotype (vs DI and II genotype) in the hypertensive and diabetic groups is associated with increased risk of hypertension and/or diabetes. OR=3.00; 95%, Cl = 0.993-9.067; OR=4.250; 95%, Cl = 1.234-14.63 respectively). The D allele was more frequent in hypertensive (77.5 %) and diabetic patients (82 %) compared to controls (52.4 %) (p=0.004 and 0.002 respectively). The D allele (vs the I allele) is associated with increased risk of hypertension and diabetes OR=3.13, 95%Cl=1.405-6.978; OR= 4.14, 95% CI= 1.615-10.622 respectively).

CONCLUSION

The DD genotype and the D allele are associated with hypertension and type 2 diabetes in Egyptian patients (Tab. 5, Fig. 1, Ref. 32).

摘要

目的

本研究旨在确定埃及人群中高血压患者和2型糖尿病（T2DM）患者血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性之间的关联。

背景

血管紧张素转换酶（ACE）基因的缺失（D）等位基因已针对高血压和2型糖尿病（T2DM）进行了研究，结果相互矛盾，这可能是由于种族和地域差异所致。

方法

共有85名受试者参与了本研究；分为高血压组（第1组）；2型糖尿病组（第2组）和对照组（第3组）。获取了每位受试者的书面知情同意书。记录每位受试者的年龄、性别、糖尿病病程和使用的药物、血压（收缩压和舒张压）以及血脂谱。通过聚合酶链反应（PCR）进行基因分型。

结果

与对照组（33.3%）相比，高血压患者（60%）和糖尿病患者（68%）的DD基因型频率显著更高（分别为p = 0.04，p = 0.01）。高血压组和糖尿病组中的DD基因型（与DI和II基因型相比）与高血压和/或糖尿病风险增加相关。OR = 3.00；95%，Cl = 0.993 - 9.067；OR = 4.250；95%，Cl分别为1.234 - 14.63）。与对照组（52.4%）相比，高血压患者（77.5%）和糖尿病患者（82%）中D等位基因更为常见（分别为p = 0.004和0.002）。D等位基因（与I等位基因相比）与高血压和糖尿病风险增加相关，OR = 3.13，95%Cl = 1.405 - 6.978；OR = 4.14，95%CI = 1.615 - 10.622）。