通过口腔微核细胞分析法对巴基斯坦古吉拉特加油站工作人员进行细胞遗传学生物监测。

Cytogenetic bio-monitoring in fuel station attendants of Gujrat, Pakistan through buccal micronucleus cytome assay.

作者信息

Butt Faiza, Cheema Kausar, Nisar Numrah, Qureshi Javed

机构信息

Lahore College for Women University, Lahore.

University of Lahore, Lahore.

出版信息

J Pak Med Assoc. 2017 Jul;67(7):1039-1044.

PMID:28770883

Abstract

OBJECTIVE

To analyse buccal epithelial cells for scoring the frequency of cytogenetic toxicity in petrol pump workers.

METHODS

The case-control study was conducted at the Lahore College for Women University, Lahore, Pakistan, from September 2014 to February 2015, and comprised petrol pump workers. Buccal micronucleus cytome assay was carried out by the collection of buccal cells after the written and oral consent of petrol pump workers. Frequencies of genotoxic (micronucleated and binucleated) and cytotoxic (karryorhetic and karyolitic) cells were scored and compared with the control group. The control group samples were collected randomly by keeping in view that these workers had not worked in petrol pumps ever. SPSS 16 was used for data analysis.

RESULTS

Of the 200 participants, there were 100(50%) workers and 100(50%) controls. Statistically significant results were observed on the comparison of frequencies of different cell anomalies in subjects compared to controls on the basis of job duration, smoking habits and age (p< 0.05 each).

CONCLUSIONS

Pump workers showed higher frequencies of cytogenetic toxicity compared to controls.

摘要

目的

分析颊黏膜上皮细胞，以评估汽油泵工作人员细胞遗传毒性的发生频率。

方法

2014年9月至2015年2月在巴基斯坦拉合尔女子大学进行了病例对照研究，研究对象为汽油泵工作人员。在获得汽油泵工作人员的书面和口头同意后，收集颊黏膜细胞进行颊黏膜微核细胞分析法检测。对遗传毒性（微核和双核）和细胞毒性（核固缩和核溶解）细胞的发生频率进行评分，并与对照组进行比较。对照组样本是在考虑到这些工作人员从未在汽油泵工作过的情况下随机收集的。使用SPSS 16进行数据分析。