Department of Obstetrics and Gynecology, People's Hospital of Linyi City, Linyi, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Jul;21(14):3169-3175.
The aim of the present study was to explore the prognostic value of long non-coding RNA CPS1-IT1 (CPS1-IT1) expression in epithelial ovarian cancer (EOC) patients and identify the effect of CPS1-IT1 on cell proliferation and apoptosis of EOC cells.
Expression levels of CPS1-IT1 in tissues and cells were detected by the Real-time quantitative RT-PCR assay. The χ2-test was used to analyze the relationship between CPS1-IT1 expression and the clinicopathological characteristics. Survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. The capacity for cellular proliferation was measured with cell counting Kit-8. Cell apoptosis assays were performed using flow cytometry. Western blot was used to detect the expression levels of cell apoptosis-related proteins.
We observed that CPS1-IT1 was significantly downregulated in EOC cell lines and tissue samples. The expression of CPS1-IT1 was significantly associated with FIGO stage and lymph node metastases. In addition, EOC patients in the low tissue CPS1-IT1 expression group had significantly shorter 5-year overall survival time than those in the high tissue CPS1-IT1 expression group. Furthermore, univariate and multivariable Cox regression analysis identified low CPS1-IT1 expression in EOC tissues as an independent poor prognostic marker of overall survival. It was also found that over-expression of CPS1-IT1 markedly promoted proliferation of EOC cells. Further studies revealed that over-expression of CPS1-IT1 induced cell apoptosis by through regulating apoptosis-related proteins.
CPS1-IT1 may be a functional tumor suppressor in EOC. It may also serve as an independent prognostic factor for patients with EOC.
本研究旨在探讨长链非编码 RNA CPS1-IT1（CPS1-IT1）表达在卵巢上皮性癌（EOC）患者中的预后价值，并确定 CPS1-IT1 对 EOC 细胞增殖和凋亡的影响。
采用实时定量 RT-PCR 检测组织和细胞中 CPS1-IT1 的表达水平。采用χ2检验分析 CPS1-IT1 表达与临床病理特征的关系。采用 Kaplan-Meier 法和 Cox 比例风险模型进行生存分析。用细胞计数试剂盒-8 检测细胞增殖能力。用流式细胞术检测细胞凋亡。用 Western blot 检测细胞凋亡相关蛋白的表达水平。
我们观察到 CPS1-IT1 在 EOC 细胞系和组织样本中显著下调。CPS1-IT1 的表达与 FIGO 分期和淋巴结转移显著相关。此外，CPS1-IT1 组织低表达组的 EOC 患者 5 年总生存率明显短于 CPS1-IT1 组织高表达组。进一步的单因素和多因素 Cox 回归分析表明，EOC 组织中 CPS1-IT1 低表达是总生存的独立预后不良因素。研究还发现，CPS1-IT1 的过表达显著促进了 EOC 细胞的增殖。进一步研究表明，CPS1-IT1 的过表达通过调节凋亡相关蛋白诱导细胞凋亡。
CPS1-IT1 可能是 EOC 中的一种功能性肿瘤抑制因子。它也可能作为 EOC 患者的独立预后因素。
