Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium.
Department of Pharmaceutical, biomedical and veterinary sciences, Laboratory of Pharmaceutical Technology and Biopharmacy, University of Antwerp, Wilrijk, Belgium.
Microb Biotechnol. 2017 Nov;10(6):1753-1763. doi: 10.1111/1751-7915.12799. Epub 2017 Aug 3.
A number of clinical studies have shown protective effects of lactobacilli against Candida species in the gastrointestinal tract, the urogenital tract and the oral cavity, while others did not show clear effects. Evidence on the mode of action of lactobacilli against Candida is also still lacking. In this study, the anti-Candida activity of the model probiotic strain Lactobacillus rhamnosus GG was explored in different assays to determine molecular interactions. We found that L. rhamnosus GG was able to interfere with Candida growth, morphogenesis and adhesion. These three aspects of Candida's physiology are all crucial to its opportunistic pathogenesis. In follow-up assays, we compared the activity of L. rhamnosus GG wild-type with its exopolysaccharide (EPS)-deficient mutant and purified EPS to evaluate the involvement of this outer carbohydrate layer. Our data demonstrate that purified EPS can both interfere with hyphal formation and adhesion to epithelial cells, which indicates that EPS is part of a combined molecular mechanism underlying the antihyphal and anti-adhesion mechanisms of L. rhamnosus GG.
许多临床研究表明，乳杆菌对胃肠道、泌尿生殖道和口腔中的念珠菌具有保护作用，但其他研究并未显示出明确的效果。关于乳杆菌对抗念珠菌作用机制的证据也仍然缺乏。在这项研究中，我们探索了模式益生菌菌株鼠李糖乳杆菌 GG 在不同试验中的抗念珠菌活性，以确定分子相互作用。我们发现，鼠李糖乳杆菌 GG 能够干扰念珠菌的生长、形态发生和黏附。这三个方面都是念珠菌机会致病的关键。在后续试验中，我们比较了鼠李糖乳杆菌 GG 野生型及其缺乏胞外多糖 (EPS) 的突变体和纯化 EPS 的活性，以评估这种外层碳水化合物层的参与情况。我们的数据表明，纯化的 EPS 既能干扰菌丝形成，又能干扰对上皮细胞的黏附，这表明 EPS 是鼠李糖乳杆菌 GG 抗菌丝和抗黏附机制的综合分子机制的一部分。
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