Haro Durand Luis A, Vargas Gabriela E, Vera-Mesones Rosa, Baldi Alberto, Zago María P, Fanovich María A, Boccaccini Aldo R, Gorustovich Alejandro
Department of Pathology and Molecular Pharmacology, IByME-CONICET, C1428ADN Buenos Aires, Argentina.
Department of Developmental Biology, National University of Salta, A4408FVY Salta, Argentina.
Materials (Basel). 2017 Jul 3;10(7):740. doi: 10.3390/ma10070740.
Since lithium (Li⁺) plays roles in angiogenesis, the localized and controlled release of Li⁺ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO₂, 24.5% Na₂O, 24.5% CaO, and 6% P₂O₅, in which Na₂O was partially substituted by 5% Li₂O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.
由于锂（Li⁺）在血管生成中发挥作用，从生物活性玻璃（BGs）中局部且可控地释放Li⁺离子代表了一种有前景的替代疗法，用于高度血管化组织的再生和修复。在此，获得了一种基于45S5 BG成分的微粒，其包含（重量百分比）45% SiO₂、24.5% Na₂O、24.5% CaO和6% P₂O₅，其中Na₂O被5% Li₂O部分替代（45S5.5Li）。结果表明，在用45S5.5Li BG的离子溶解产物（IDPs）刺激后，人脐静脉内皮细胞（HUVECs）在体外具有更强的迁移和增殖反应以及形成小管的能力。结果还显示了经典Wnt/β-连环蛋白途径的激活以及促血管生成细胞因子胰岛素样生长因子1（IGF1）和转化生长因子β（TGFβ）表达的增加。我们得出结论，45S5.5Li BG的IDPs将作为有用的无机剂来改善组织修复和再生，最终在无外源性生长因子的情况下刺激HUVECs的行为。
