Kanematsu Hideyuki, Kudara Hikonaru, Kanesaki Shun, Kogo Takeshi, Ikegai Hajime, Ogawa Akiko, Hirai Nobumitsu
Department of Materials Science and Engineering, National Institute of Technology, Suzuka College, Suzuka Mie 510-0294, Japan.
Department of Chemistry and Biochemistry, National Institute of Technology, Suzuka College, Suzuka Mie 510-0294, Japan.
Materials (Basel). 2016 Oct 11;9(10):824. doi: 10.3390/ma9100824.
A laboratory biofilm reactor (LBR) was modified to a new loop-type closed system in order to evaluate novel stents and catheter materials using 3D optical microscopy and Raman spectroscopy. Two metallic specimens, pure nickel and cupronickel (80% Cu-20% Ni), along with two polymers, silicone and polyurethane, were chosen as examples to ratify the system. Each set of specimens was assigned to the LBR using either tap water or an NB (Nutrient broth based on peptone from animal foods and beef extract mainly)-cultured solution with formed over 48-72 h. The specimens were then analyzed using Raman Spectroscopy. 3D optical microscopy was employed to corroborate the Raman Spectroscopy results for only the metallic specimens since the inherent roughness of the polymer specimens made such measurements difficult. The findings suggest that the closed loop-type LBR together with Raman spectroscopy analysis is a useful method for evaluating biomaterials as a potential urinary system.
为了使用三维光学显微镜和拉曼光谱评估新型支架和导管材料，将实验室生物膜反应器（LBR）改装为一种新型的循环式封闭系统。选择了两种金属样本，纯镍和白铜（80%铜 - 20%镍），以及两种聚合物，硅酮和聚氨酯，作为验证该系统的示例。每组样本使用自来水或基于动物食品蛋白胨和牛肉提取物的营养肉汤（NB）培养溶液分配到LBR中，在48 - 72小时内形成生物膜。然后使用拉曼光谱对样本进行分析。由于聚合物样本固有的粗糙度使得此类测量困难，仅对金属样本采用三维光学显微镜来证实拉曼光谱的结果。研究结果表明，闭环式LBR与拉曼光谱分析相结合是评估作为潜在泌尿系统生物材料的一种有用方法。
