Kamimura Ryuto, Kanematsu Hideyuki, Ogawa Akiko, Kogo Takeshi, Miura Hidekazu, Kawai Risa, Hirai Nobumitsu, Kato Takehito, Yoshitake Michiko, Barry Dana M
National Institute of Technology (KOSEN), Suzuka College, Suzuka 510-0294, Mie, Japan.
Faculty of Medical Engineering, Suzuka University of Medical Science, Suzuka 510-0293, Mie, Japan.
Materials (Basel). 2022 Sep 28;15(19):6727. doi: 10.3390/ma15196727.
Biofilms have caused many problems, not only in the industrial fields, but also in our daily lives. Therefore, it is important for us to control them by evaluating them properly. There are many instrumental analytical methods available for evaluating formed biofilm qualitatively. These methods include the use of Raman spectroscopy and various microscopes (optical microscopes, confocal laser microscopes, scanning electron microscopes, transmission electron microscopes, atomic force microscopes, etc.). On the other hand, there are some biological methods, such as staining, gene analyses, etc. From the practical viewpoint, staining methods seem to be the best due to various reasons. Therefore, we focused on the staining method that used a crystal violet solution. In the previous study, we devised an evaluation process for biofilms using a color meter to analyze the various staining situations. However, this method was complicated and expensive for practical engineers. For this experiment, we investigated the process of using regular photos that were quantified without any instruments except for digitized cameras. Digitized cameras were used to compare the results. As a result, we confirmed that the absolute values were different for both cases, respectively. However, the tendency of changes was the same. Therefore, we plan to utilize the changes before and after biofilm formation as indicators for the future.
生物膜引发了诸多问题，不仅在工业领域，在我们的日常生活中亦是如此。因此，通过恰当评估来控制生物膜对我们而言至关重要。有许多仪器分析方法可用于定性评估已形成的生物膜。这些方法包括使用拉曼光谱法以及各种显微镜（光学显微镜、共聚焦激光显微镜、扫描电子显微镜、透射电子显微镜、原子力显微镜等）。另一方面，也有一些生物学方法，比如染色、基因分析等。从实际角度来看，由于各种原因，染色方法似乎是最佳选择。因此，我们聚焦于使用结晶紫溶液的染色方法。在之前的研究中，我们设计了一个使用色差仪分析各种染色情况来评估生物膜的过程。然而，对于实际工程师而言，这种方法既复杂又昂贵。对于本实验，我们研究了使用普通照片的过程，除了数码照相机外无需任何仪器即可对其进行量化。使用数码照相机来比较结果。结果，我们确认两种情况下的绝对值分别不同。然而，变化趋势是相同的。因此，我们计划将生物膜形成前后的变化用作未来的指标。
