Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopolis, BP. 901, 2050 Hammam-Lif, Tunisia.
Laboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj-Cedria Technopolis, BP. 901, 2050 Hammam-Lif, Tunisia.
Food Res Int. 2017 Sep;99(Pt 1):702-712. doi: 10.1016/j.foodres.2017.06.009. Epub 2017 Jun 3.
In this work, LC-ESI-TOF-MS and GC-EI-MS were used to assess the potential of Artemisia herba alba as a source of health-promoting constituents. Besides, the antioxidant, the antimicrobial and the cytotoxic potentials were evaluated. A total of 86 metabolites, including C-glycosylated and methylated flavones, quinic acid derivatives, coumarins, sesquiterpenes lactones, terpenoids, fatty acids, carbohydrates, organic acids and alkaloids were identified, sixty five of them were reported for the first time in A. herba alba. The main compounds were di-O-caffeoylquinic acids, artemisinic acid, menthol, α-ketoglutaric acid, scopolin, isoschaftoside and sucrose. Furthermore, results showed that A. herba alba possess high total antioxidant activity (Total antioxidant activity=423mg gallic acid equivalent/g dry weight), strong potential anticancer capacity against MCF-7 breast cancer and HeLa human cervical cell lines (IC of 15 and 70μg/ml, respectively), and moderate antibacterial activity against S. aureus, B. thurigiensis and A. hydrophila. These results support the use of this plant as alternative bioactive ingredient for functional foods, dietary supplements or nutraceuticals.
在这项工作中，我们使用 LC-ESI-TOF-MS 和 GC-EI-MS 来评估青蒿作为具有促进健康成分的潜在来源。此外，还评估了其抗氧化、抗菌和细胞毒性潜力。总共鉴定出 86 种代谢物，包括 C-糖基化和甲基化的类黄酮、奎尼酸衍生物、香豆素、倍半萜内酯、萜类、脂肪酸、碳水化合物、有机酸和生物碱，其中 65 种代谢物是首次在青蒿中发现的。主要化合物为二-O-咖啡酰奎宁酸、青蒿酸、薄荷醇、α-酮戊二酸、水苏碱、异荭草苷和蔗糖。此外，研究结果表明，青蒿具有较高的总抗氧化活性（总抗氧化活性=423mg 没食子酸当量/g 干重），对 MCF-7 乳腺癌和 HeLa 人宫颈细胞系具有较强的抗癌潜力（IC 分别为 15 和 70μg/ml），对金黄色葡萄球菌、苏云金芽孢杆菌和嗜水气单胞菌具有中等的抗菌活性。这些结果支持将该植物作为功能性食品、膳食补充剂或营养保健品的替代生物活性成分使用。
