Gharbi S, Bally M, Lazdunski A, Murgier M
Biochimie. 1986 Oct-Nov;68(10-11):1159-63. doi: 10.1016/s0300-9084(86)80059-1.
Mutants altered in their expression of the hybrid pepN-lacZ gene have been selected for resistance to p-nitrophenyl-beta-D-thiogalactopyranoside (a bacteriostatic compound that enters the cells via lac permease). A unique mutation decreasing the level of pepN expression to 9% of that of the wild type has been studied in detail. This mutation controls in cis the expression of the pepN gene. The pepN region from a pepN-lacZ gene fusion has been cloned and sequenced. Comparison of the mutant and wild type sequences indicates that the mutation lies between the Shine-Dalgarno sequence (AGGT) and the initiation codon (AUG). This mutation is a T----C transition which might allow the formation of a stable secondary structure in the region of translation initiation thus decreasing the level of pepN expression.
已筛选出杂交pepN - lacZ基因表达发生改变的突变体，使其对对硝基苯基 - β - D - 硫代半乳糖苷（一种通过乳糖通透酶进入细胞的抑菌化合物）具有抗性。已详细研究了一种独特的突变，该突变使pepN表达水平降至野生型的9%。此突变顺式调控pepN基因的表达。已克隆并测序了来自pepN - lacZ基因融合体的pepN区域。突变体与野生型序列的比较表明，该突变位于Shine - Dalgarno序列（AGGT）和起始密码子（AUG）之间。此突变是一个T→C转换，可能会在翻译起始区域形成稳定的二级结构，从而降低pepN的表达水平。
