Bozouklian H, Elmerich C
Biochimie. 1986 Oct-Nov;68(10-11):1181-7. doi: 10.1016/s0300-9084(86)80062-1.
The complete nucleotide sequence of the glnA gene, encoding the glutamine synthetase subunit of Azospirillum brasilense Sp7, was established. This is the first Azospirillum gene sequenced. The gene encodes a 468 residue polypeptide of MW 51,917. The similarity coefficient (SAB) between the polypeptidic sequence of Azospirillum and Anabaena 7120, which is the only other glnA sequence available, is 58%. No significant homology with E. coli canonical and ntr promoters, or with the promoter region of the Anabaena glnA gene was found. When fused to an E. coli promoter, the gene could be translated in E. coli, despite a very biased codon usage and an atypical Shine-Dalgarno sequence.
确定了编码巴西固氮螺菌Sp7谷氨酰胺合成酶亚基的glnA基因的完整核苷酸序列。这是第一个被测序的固氮螺菌基因。该基因编码一个由468个残基组成、分子量为51,917的多肽。固氮螺菌与鱼腥藻7120（唯一可获得的另一个glnA序列）的多肽序列之间的相似系数（SAB）为58%。未发现与大肠杆菌标准启动子和ntr启动子或鱼腥藻glnA基因的启动子区域有明显同源性。当与大肠杆菌启动子融合时，尽管密码子使用非常偏向且有一个非典型的Shine-Dalgarno序列，该基因仍能在大肠杆菌中翻译。
