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嗜酸自养型氧化亚铁硫杆菌谷氨酰胺合成酶基因及其调控区的核苷酸序列

Nucleotide sequence of the glutamine synthetase gene and its controlling region from the acidophilic autotroph Thiobacillus ferrooxidans.

作者信息

Rawlings D E, Jones W A, O'Neill E G, Woods D R

出版信息

Gene. 1987;53(2-3):211-7. doi: 10.1016/0378-1119(87)90009-6.

DOI:10.1016/0378-1119(87)90009-6
PMID:2886400
Abstract

A 2089-bp chromosomal DNA segment containing the Thiobacillus ferrooxidans glnA gene has been sequenced. Putative glnAp1-type promoter sequences, a consensus ntrC-gene-product-binding site and a catabolite-activating protein consensus recognition sequence were detected upstream of the structural gene. The glnA gene was followed by a sequence resembling a Rho-independent termination sequence. The complete amino acid sequence (468 residues) of the glutamine synthetase (GS) has been deduced, and comparisons are made with reported amino acid sequences of GS from other organisms.

摘要

已对一段包含氧化亚铁硫杆菌谷氨酰胺合成酶基因(glnA)的2089碱基对的染色体DNA片段进行了测序。在结构基因上游检测到了假定的glnAp1型启动子序列、一个共有ntrC基因产物结合位点和一个分解代谢物激活蛋白共有识别序列。glnA基因后面跟着一段类似于不依赖Rho的终止序列的序列。已推导得到谷氨酰胺合成酶(GS)的完整氨基酸序列(468个残基),并与其他生物体中报道的GS氨基酸序列进行了比较。

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