Guedes Duschinka Rd, Paiva Marcelo Hs, Donato Mariana Ma, Barbosa Priscilla P, Krokovsky Larissa, Rocha Sura W Dos S, Saraiva Karina LA, Crespo Mônica M, Rezende Tatiana Mt, Wallau Gabriel L, Barbosa Rosângela Mr, Oliveira Cláudia Mf, Melo-Santos Maria Av, Pena Lindomar, Cordeiro Marli T, Franca Rafael F de O, Oliveira André Ls de, Peixoto Christina A, Leal Walter S, Ayres Constância Fj
Departamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz), Recife 50760-420, Brazil.
Núcleo de Ciências da Vida, Centro Acadêmico do Agreste, Universidade Federal de Pernambuco, Caruaru 55002-970, Brazil.
Emerg Microbes Infect. 2017 Aug 9;6(8):e69. doi: 10.1038/emi.2017.59.
Zika virus (ZIKV) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. The virus is primarily transmitted by mosquito bite, although other routes of infection have been implicated in some cases. The Aedes aegypti mosquito is considered to be the main vector to humans worldwide; however, there is evidence that other mosquito species, including Culex quinquefasciatus, transmit the virus. To test the potential of Cx. quinquefasciatus to transmit ZIKV, we experimentally compared the vector competence of laboratory-reared Ae. aegypti and Cx. quinquefasciatus. Interestingly, we were able to detect the presence of ZIKV in the midgut, salivary glands and saliva of artificially fed Cx. quinquefasciatus. In addition, we collected ZIKV-infected Cx. quinquefasciatus from urban areas with high microcephaly incidence in Recife, Brazil. Corroborating our experimental data from artificially fed mosquitoes, ZIKV was isolated from field-caught Cx. quinquefasciatus, and its genome was partially sequenced. Collectively, these findings indicate that there may be a wider range of ZIKV vectors than anticipated.
寨卡病毒（ZIKV）是一种黄病毒，最近它与新生儿小头畸形及其他神经疾病发病率的上升有关。该病毒主要通过蚊虫叮咬传播，不过在某些情况下其他感染途径也有涉入。埃及伊蚊被认为是全球人类的主要传播媒介；然而，有证据表明包括致倦库蚊在内的其他蚊种也能传播该病毒。为了测试致倦库蚊传播寨卡病毒的可能性，我们通过实验比较了实验室饲养的埃及伊蚊和致倦库蚊的媒介能力。有趣的是，我们能够在人工喂食的致倦库蚊的中肠、唾液腺和唾液中检测到寨卡病毒的存在。此外，我们从巴西累西腓小头畸形发病率高的城市地区采集到了感染寨卡病毒的致倦库蚊。从野外捕获的致倦库蚊中分离出了寨卡病毒，并对其基因组进行了部分测序，这证实了我们从人工喂食蚊子获得的实验数据。总的来说，这些发现表明寨卡病毒的传播媒介范围可能比预期的更广。
