Agronomy College/National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, 450002, China.
Sci Rep. 2017 Aug 8;7(1):7524. doi: 10.1038/s41598-017-08069-9.
By comparing the differentially accumulated proteins from the derivatives (UC 1110 × PI 610750) in the F recombinant inbred line population which differed in cold-tolerance, altogether 223 proteins with significantly altered abundance were identified. The comparison of 10 cold-sensitive descendant lines with 10 cold-tolerant descendant lines identified 140 proteins that showed decreased protein abundance, such as the components of the photosynthesis apparatus and cell-wall metabolism. The identified proteins were classified into the following main groups: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, sulfur metabolism, nitrogen metabolism, RNA metabolism, energy production, cell-wall metabolism, membrane and transportation, and signal transduction. Results of quantitative real-time PCR of 20 differentially accumulated proteins indicated that the transcriptional expression patterns of 10 genes were consistent with their protein expression models. Virus-induced gene silencing of Hsp90, BBI, and REP14 genes indicated that virus-silenced plants subjected to cold stress had more severe drooping and wilting, an increased rate of relative electrolyte leakage, and reduced relative water content compared to viral control plants. Furthermore, ultrastructural changes of virus-silenced plants were destroyed more severely than those of viral control plants. These results indicate that Hsp90, BBI, and REP14 potentially play vital roles in conferring cold tolerance in bread wheat.
通过比较在冷耐性不同的 F 重组近交系群体中 UC 1110×PI 610750 的衍生物中差异积累的蛋白质，总共鉴定出 223 种丰度明显改变的蛋白质。将 10 个冷敏感后代系与 10 个冷耐受后代系进行比较，鉴定出 140 种蛋白质的蛋白丰度降低，如光合作用器和细胞壁代谢的组成部分。鉴定出的蛋白质被分类为以下主要组：蛋白质代谢、应激/防御、碳水化合物代谢、脂质代谢、硫代谢、氮代谢、RNA 代谢、能量产生、细胞壁代谢、膜和运输以及信号转导。20 种差异积累蛋白的实时定量 PCR 结果表明，10 个基因的转录表达模式与其蛋白表达模式一致。Hsp90、BBI 和 REP14 基因的病毒诱导基因沉默表明，与病毒对照植物相比，冷胁迫下的病毒沉默植物表现出更严重的下垂和萎蔫、相对电解质泄漏率增加和相对含水量降低。此外，与病毒对照植物相比，病毒沉默植物的超微结构变化被破坏得更严重。这些结果表明，Hsp90、BBI 和 REP14 可能在赋予小麦耐寒性方面发挥重要作用。
