Hou Chunxi, Guan Shuwen, Wang Ruidi, Zhang Wei, Meng Fanchao, Zhao Linlu, Xu Jiayun, Liu Junqiu
Department of Chemistry, University of British Columbia , Vancouver, British Columbia V6T 1Z1, Canada.
Zhuhai United Laboratories Co., Ltd. , Nation High & New Technology Industry Development Zone, Zhuhai 519040, China.
J Phys Chem Lett. 2017 Sep 7;8(17):3970-3979. doi: 10.1021/acs.jpclett.7b01564. Epub 2017 Aug 10.
DNA plays an important role in the process of protein assembly. DNA viruses such as the M13 virus are typical examples in which single DNA genomes behave as templates to induce the assembly of multiple major coat protein (PVIII) monomers. Thus, the design of protein assemblies based on DNA templates attracts much interest in the construction of supramolecular structures and materials. With the development of DNA nanotechnology, precise 1D and 3D protein nanostructures have been designed and constructed by using DNA templates through DNA-protein interactions, protein-ligand interactions, and protein-adapter interactions. These DNA-templated protein assemblies show great potential in catalysis, medicine, light-responsive systems, drug delivery, and signal transduction. Herein, we review the progress on DNA-based protein nanostructures that possess sophisticated nanometer-sized structures with programmable shapes and stimuli-responsive parameters, and we present their great potential in the design of biomaterials and biodevices in the future.
DNA在蛋白质组装过程中起着重要作用。诸如M13病毒之类的DNA病毒就是典型例子，其中单个DNA基因组充当模板，诱导多个主要衣壳蛋白（PVIII）单体的组装。因此，基于DNA模板的蛋白质组装设计在超分子结构和材料构建方面引起了广泛关注。随着DNA纳米技术的发展，通过DNA-蛋白质相互作用、蛋白质-配体相互作用和蛋白质-衔接子相互作用，利用DNA模板设计并构建了精确的一维和三维蛋白质纳米结构。这些基于DNA模板的蛋白质组装在催化、医学、光响应系统、药物递送和信号转导方面显示出巨大潜力。在此，我们综述了具有复杂纳米尺寸结构、可编程形状和刺激响应参数的基于DNA的蛋白质纳米结构的研究进展，并展示了它们在未来生物材料和生物器件设计中的巨大潜力。
