Morris J L, Gibbins I L, Murphy R
Neurosci Lett. 1986 Nov 21;71(3):264-70. doi: 10.1016/0304-3940(86)90631-2.
Noradrenergic perivascular axons were demonstrated in all systemic arteries and veins of a marsupial, the brush-tailed possum (Trichosurus vulpecula), by a catecholamine fluorescence procedure and with antisera directed against the catecholamine-synthesizing enzymes tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (D beta H). Perivascular axons with neuropeptide Y-like immunoreactivity (NPY-LI) were not found in most systemic arteries and veins using antisera which recognize NPY and other members of the pancreatic polypeptide family in diverse vertebrate species. The exceptions were the renal, coeliac, main mesenteric and iliac arteries, where up to 50% of axons with TH-LI or D beta H-LI also showed NPY-LI with two of the 4 antisera used. No noradrenergic nerve cell bodies in thoracic sympathetic ganglia had NPY-LI, whilst 3% of noradrenergic nerve cell bodies in lumbar sympathetic chain ganglia had weak NPY-LI. This marsupial is the first vertebrate species found to date in which the majority of perivascular noradrenergic axons do not contain NPY-LI. If these axons contain an as yet unidentified neuropeptide, it is unlikely to be closely related to NPY.
通过儿茶酚胺荧光法以及针对儿茶酚胺合成酶酪氨酸羟化酶（TH）和多巴胺-β-羟化酶（DβH）的抗血清，在有袋动物刷尾负鼠（Trichosurus vulpecula）的所有全身动脉和静脉中都证实了去甲肾上腺素能血管周围轴突。使用能识别多种脊椎动物物种中神经肽Y（NPY）和胰腺多肽家族其他成员的抗血清，在大多数全身动脉和静脉中未发现具有神经肽Y样免疫反应性（NPY-LI）的血管周围轴突。例外情况是肾动脉、腹腔动脉、肠系膜上动脉和髂动脉，在这些动脉中，使用的4种抗血清中有2种显示，高达50%的具有TH-LI或DβH-LI的轴突也显示NPY-LI。胸交感神经节中没有去甲肾上腺素能神经细胞体具有NPY-LI，而腰交感链神经节中3%的去甲肾上腺素能神经细胞体具有微弱的NPY-LI。这种有袋动物是迄今为止发现的第一种大多数血管周围去甲肾上腺素能轴突不含有NPY-LI的脊椎动物物种。如果这些轴突含有一种尚未确定的神经肽，那么它不太可能与NPY密切相关。
