Wang Kaikai, Wang Juan, Hu Wenwen, Zhang Yifan, Zhi Feng, Zhou Zaigang, Wu Jinhui, Hu Yiqiao
State Key laboratory of Pharmaceutical Biotechnology & Medical School, Nanjing University, Nanjing 210093, China.
Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
Materials (Basel). 2015 Dec 11;8(12):8701-8713. doi: 10.3390/ma8125486.
Hemoglobin is a promising drug carrier but lacks extensive investigation. The chemical conjugation of hemoglobin and drugs is costly and complex, so we have developed curcumin-loaded hemoglobin nanoparticles (CCM-Hb-NPs) via self-assembly for the first time. Using the acid-denaturing method, we avoid introducing denaturants and organic solvents. The nanoparticles are stable with uniform size. We have conducted a series of experiments to examine the interaction of hemoglobin and CCM, including hydrophobic characterization, SDS-PAGE. These experiments substantiate that this self-assembly process is mainly driven by hydrophobic forces. Our nanoparticles achieve much higher cell uptake efficiency and cytotoxicity than free CCM solution . The uptake inhibition experiments also demonstrate that our nanoparticles were incorporated via the classic clathrin-mediated endocytosis pathway. These results indicate that hemoglobin nanoparticles formed by self-assembly are a promising drug delivery system for cancer therapy.
血红蛋白是一种很有前景的药物载体，但缺乏广泛的研究。血红蛋白与药物的化学偶联成本高且复杂，因此我们首次通过自组装开发了负载姜黄素的血红蛋白纳米颗粒（CCM-Hb-NPs）。使用酸变性方法，我们避免引入变性剂和有机溶剂。纳米颗粒稳定且尺寸均匀。我们进行了一系列实验来研究血红蛋白与CCM的相互作用，包括疏水特性、SDS-PAGE。这些实验证实这种自组装过程主要由疏水力驱动。我们的纳米颗粒比游离CCM溶液具有更高的细胞摄取效率和细胞毒性。摄取抑制实验还表明我们的纳米颗粒是通过经典的网格蛋白介导的内吞途径被摄取的。这些结果表明，通过自组装形成的血红蛋白纳米颗粒是一种很有前景的癌症治疗药物递送系统。
