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从一个经流式细胞分选的人类8号染色体文库中分离并鉴定出可检测限制性片段长度多态性（RFLP）的DNA探针。

Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP).

作者信息

Wood S, Starr T V, Shukin R J

出版信息

Am J Hum Genet. 1986 Dec;39(6):744-50.

PMID:2879441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1684124/
Abstract

We have used a recombinant DNA library constructed from flow-sorted human chromosome 8 as a source of single-copy human probes. These probes have been screened for restriction fragment length polymorphism (RFLP) by hybridization to Southern transfers of genomic DNA from five unrelated individuals. We have detected six RFLPs distributed among four probes after screening 741 base pairs for restriction site variation. These RFLPs all behave as codominant Mendelian alleles. Two of the probes detect rare variants, while the other two detect RFLPs with PIC values of .36 and .16. Informative probes will be useful for the construction of a linkage map for chromosome 8 and for the localization of mutant alleles to this chromosome.

摘要

我们使用了一个由流式分选的人类8号染色体构建的重组DNA文库作为单拷贝人类探针的来源。通过与来自五个无关个体的基因组DNA的Southern印迹杂交，对这些探针进行了限制性片段长度多态性（RFLP）筛选。在筛选741个碱基对的限制性位点变异后，我们在四个探针中检测到六个RFLP。这些RFLP均表现为共显性孟德尔等位基因。其中两个探针检测到罕见变异，而另外两个探针检测到PIC值分别为0.36和0.16的RFLP。信息丰富的探针将有助于构建8号染色体的连锁图谱，并将突变等位基因定位到该染色体上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d69/1684124/10e3a5d9dbf8/ajhg00148-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d69/1684124/10e3a5d9dbf8/ajhg00148-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d69/1684124/10e3a5d9dbf8/ajhg00148-0071-a.jpg

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引用本文的文献

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本文引用的文献

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