Watkins P C, Tanzi R E, Gibbons K T, Tricoli J V, Landes G, Eddy R, Shows T B, Gusella J F
Nucleic Acids Res. 1985 Sep 11;13(17):6075-88. doi: 10.1093/nar/13.17.6075.
A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction fragment length polymorphisms to provide genetic markers on chromosome 21. Nine independently isolated clones used as probes identified a total of 11 new RFLPs. Four of the DNA probes recovered from the library have been mapped unequivocally to chromosome 21 using a panel of somatic cell hybrid lines. A fifth probe detected an RFLP on chromosome 21 as well as sequences on other chromosomes. This set of RFLPs may now form the basis for construction of a genetic linkage map of human chromosome 21.
一种以小鼠为背景、仅包含人类21号染色体的体细胞杂交系已被用作构建重组噬菌体文库的DNA来源。已鉴定出含有人类插入片段的单个噬菌体。已制备了无重复的人类DNA亚克隆，并用于筛选限制性片段长度多态性，以提供21号染色体上的遗传标记。用作探针的9个独立分离的克隆共鉴定出11个新的RFLP。从文库中回收的4个DNA探针已使用一组体细胞杂交系明确地定位到21号染色体上。第五个探针在21号染色体以及其他染色体上检测到RFLP。这组RFLP现在可能构成构建人类21号染色体遗传连锁图谱的基础。
