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卢旺达鲁巴武和尼亚比胡地区奶牛场亚临床乳腺炎的患病率及病原菌分布

Prevalence of Subclinical Mastitis and Distribution of Pathogens in Dairy Farms of Rubavu and Nyabihu Districts, Rwanda.

作者信息

Mpatswenumugabo J P, Bebora L C, Gitao G C, Mobegi V A, Iraguha B, Kamana O, Shumbusho B

机构信息

Department of Veterinary Medicine, University of Rwanda, P.O. Box 210, Musanze, Rwanda.

Department of Veterinary Pathology, Microbiology and Parasitology, University of Nairobi, P.O. Box 29053-00625, Kangemi, Kenya.

出版信息

J Vet Med. 2017;2017:8456713. doi: 10.1155/2017/8456713. Epub 2017 Jul 17.

Abstract

A cross-sectional study was conducted from May 2016 to January 2017 in Rubavu and Nyabihu districts, Western Rwanda, aiming at estimating the prevalence of subclinical mastitis (SCM) and identifying its causative bacteria. Management practices and milking procedures were recorded through a questionnaire. 123 crossbreed milking cows from 13 dairy farms were randomly selected and screened for SCM using California Mastitis Test (CMT). Composite CMT positive milk samples were processed for bacterial isolation and identification. The overall SCM prevalence at cow level was 50.4%. 68 bacterial isolates were identified by morphological and biochemical characteristics. They included, Coagulase Negative Staphylococci (51.5%), (20.6%), species (10.3%), species (10.3%), (5.8%), and (1.5%). About 67.1% of the farmers checked for mastitis; of these, 58.9% relied on clinical signs and only 6.8% screened with CMT. Only 5.5% and 2.7% of the farmers tried to control mastitis using dry cow therapy and teat dips, respectively. Thus, to reduce the prevalence of SCM, farmers in the study area need to be trained on good milking practices, including regular use of teat dips, application of dry cow therapy, and SCM screening. This will improve their sales and their financial status.

摘要

2016年5月至2017年1月,在卢旺达西部的鲁巴武和尼亚比胡地区开展了一项横断面研究,旨在评估亚临床型乳腺炎(SCM)的患病率并确定其致病细菌。通过问卷调查记录管理措施和挤奶程序。从13个奶牛场随机选取123头杂交泌乳奶牛,使用加利福尼亚乳腺炎检测法(CMT)对其进行SCM筛查。对CMT检测呈阳性的混合乳样进行细菌分离和鉴定。奶牛层面的总体SCM患病率为50.4%。通过形态学和生化特征鉴定出68株细菌分离株。其中包括凝固酶阴性葡萄球菌(51.5%)、 (20.6%)、 种(10.3%)、 种(10.3%)、 (5.8%)和 (1.5%)。约67.1%的奶农接受了乳腺炎检查;其中,58.9%依靠临床症状判断,仅有6.8%使用CMT进行筛查。分别仅有5.5%和2.7%的奶农尝试采用干奶期治疗和乳头药浴来控制乳腺炎。因此,为降低SCM的患病率,研究区域的奶农需要接受关于良好挤奶操作的培训,包括定期使用乳头药浴、应用干奶期治疗以及进行SCM筛查。这将提高他们的销售量和财务状况。

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