印度尼西亚茂物奶牛中分离出的耐甲氧西林相关亚临床乳腺炎的鉴定及药敏试验
Identification and antimicrobial susceptibility of methicillin-resistant -associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia.
作者信息
Qolbaini Evi Nur, Khoeri Miftahudin Majid, Salsabila Korrie, Paramaiswari Wisiva Tofriska, Tafroji Wisnu, Artika I Made, Safari Dodi
机构信息
Department of Biochemistry, IPB University, Jl. Raya Dramaga, Bogor, 16680, Indonesia.
Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.
出版信息
Vet World. 2021 May;14(5):1180-1184. doi: 10.14202/vetworld.2021.1180-1184. Epub 2021 May 13.
Abstract
BACKGROUND AND AIM
Subclinical mastitis is an udder infection devoid of clinical symptoms, and is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant (MRSA)-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia.
MATERIALS AND METHODS
was isolated from subclinical mastitis milk specimens. All strains were confirmed by polymerase chain reaction-based detection of staphylococcal , and MRSA was confirmed by the presence of . Antibiotic susceptibility was determined using the disk diffusion method.
RESULTS
From 86 milk samples, 49 isolates (57%) were confirmed as . All isolates were susceptible to tetracycline, gentamicin, chloramphenicol, erythromycin, and trimethoprim/sulfamethoxazole. Nine isolates were identified as MRSA (10.5%).
CONCLUSION
In this study, we reported MRSA-associated subclinical mastitis in Bogor, Indonesia.
摘要
背景与目的
亚临床型乳腺炎是一种无临床症状的乳房感染疾病，且是引发该疾病的细菌之一。本研究旨在鉴定并确定从印度尼西亚茂物的奶牛中分离出的耐甲氧西林金黄色葡萄球菌（MRSA）相关亚临床型乳腺炎的患病率及抗生素敏感性。
材料与方法
从亚临床型乳腺炎乳汁样本中分离出[具体物质未给出]。所有菌株通过基于聚合酶链反应的葡萄球菌[具体物质未给出]检测得以确认，而MRSA通过[具体物质未给出]的存在得以确认。使用纸片扩散法测定抗生素敏感性。
结果
从86份乳汁样本中，49株分离株（57%）被确认为[具体物质未给出]。所有分离株对四环素、庆大霉素、氯霉素、红霉素和甲氧苄啶/磺胺甲恶唑敏感。9株分离株被鉴定为MRSA（10.5%）。
结论
在本研究中，我们报告了印度尼西亚茂物存在MRSA相关亚临床型乳腺炎。
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