Essel Leslie B, Obiri David D, Osafo Newman, Antwi Aaron O, Duduyemi Babatunde M
Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology (KNUST), Kumasi, Ghana.
Department of Pathology, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology (KNUST), Kumasi, Ghana.
Int Sch Res Notices. 2017 Jul 17;2017:6859230. doi: 10.1155/2017/6859230. eCollection 2017.
We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was assessed for damage. Lung tissue homogenates were assayed for glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO). Phytochemical analysis was carried out on the stem bark. Acute toxicity studies were conducted in rats. In the pleurisy model the extract (30-300 mg/kg) significantly reduced the volume and amount of proteins and leucocytes in the exudate and also protected against lung injury. Tissue level of GSH and SOD and CAT expression were increased while MDA level and MPO activity were reduced. The peak and total oedema responses were significantly suppressed when given both preemptively and curatively in the mice paw oedema test. Saponins, alkaloids, triterpenoids, and tannins were present in the stem bark. extract exhibited no apparent acute toxicity. We conclude that the ethanolic stem-bark extract of has antioxidant action and exhibits significant anti-inflammatory activity through suppression of pleurisy and paw oedema induced with carrageenan.
我们研究了[植物名称]茎皮70%（v/v）乙醇提取物对角叉菜胶诱导的小鼠胸膜炎和爪肿胀模型的抗氧化和抗炎作用。分析大鼠胸腔积液的体积、蛋白质含量和白细胞数量，同时评估肺组织学损伤情况。检测肺组织匀浆中的谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和髓过氧化物酶（MPO）。对茎皮进行植物化学分析。在大鼠中进行急性毒性研究。在胸膜炎模型中，提取物（30 - 300mg/kg）显著降低了渗出液中蛋白质和白细胞的体积和数量，并对肺损伤起到保护作用。组织中GSH水平、SOD和CAT表达增加，而MDA水平和MPO活性降低。在小鼠爪肿胀试验中，无论是预防性还是治疗性给药，提取物均显著抑制了峰值和总水肿反应。茎皮中存在皂苷、生物碱、三萜类化合物和单宁。[植物名称]提取物未表现出明显的急性毒性。我们得出结论，[植物名称]的乙醇茎皮提取物具有抗氧化作用，并通过抑制角叉菜胶诱导的胸膜炎和爪肿胀表现出显著的抗炎活性。
