Guangxi Biological Polysaccharide Separation, Purification and Modification Research Platform, Guangxi University for Nationalities, Nanning 530006, China.
School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China.
Biosensors (Basel). 2021 Apr 22;11(5):130. doi: 10.3390/bios11050130.
In this work, a novel fluorescent probe with first-time-selected thiazepine backbone, TZPzine-1, was developed for selective detection of hydrazine in water samples and living cells. Chosen from our recent anti-cancer agents, TZPzine-1 inferred structurally based advantages of the optical adjustability and the hydrazine-trapping approach. It also showed applicable properties including high sensitivity (LOD = 50 nM), wide linear range (0-15 equiv.), high selectivity (especially from competing species), rapid response (within 20 min), and practical steadiness in various pH (6.0-11.0) and temperature (15-50 °C) conditions. To satisfy the interdisciplinary requirements in environmental toxicology, TZPzine-1 was successfully applied in water samples and living cells. We hope that the information in this work, as well as the concept of monitoring the nitrogen cycle, may be referable for future research on systematic management.
在这项工作中，我们开发了一种新型的基于噻唑烷骨架的荧光探针 TZPzine-1，用于选择性检测水样和活细胞中的肼。TZPzine-1 是从我们最近的抗癌剂中选择的，它推断出基于结构的光学可调性和肼捕获方法的优势。它还具有适用的特性，包括高灵敏度（LOD=50 nM）、宽线性范围（0-15 当量）、高选择性（特别是与竞争物种相比）、快速响应（在 20 分钟内）以及在各种 pH（6.0-11.0）和温度（15-50°C）条件下的实际稳定性。为了满足环境毒理学中的跨学科要求，TZPzine-1 已成功应用于水样和活细胞中。我们希望这项工作中的信息以及监测氮循环的概念，可为系统管理的未来研究提供参考。
