Savoldi Robson, Polari Daniel, Pinheiro-da-Silva Jaquelinne, Silva Priscila F, Lobao-Soares Bruno, Yonamine Mauricio, Freire Fulvio A M, Luchiari Ana C
Luchiari Lab, Physiology, Federal University of Rio Grande do NorteNatal, Brazil.
Biophysics and Pharmacology, Federal University of Rio Grande do NorteNatal, Brazil.
Front Behav Neurosci. 2017 Jul 28;11:139. doi: 10.3389/fnbeh.2017.00139. eCollection 2017.
The combined infusion of stem and leaves, known as ayahuasca, has been used for centuries by indigenous tribes. The infusion is rich in , -dimethyltryptamine (DMT) and monoamine oxidase inhibitors, with properties similar to those of serotonin. Despite substantial progress in the development of new drugs to treat anxiety and depression, current treatments have several limitations. Alternative drugs, such as ayahuasca, may shed light on these disorders. Here, we present time-course behavioral changes induced by ayahuasca in zebrafish, as first step toward establishing an ideal concentration for pre-clinical evaluations. We exposed adult zebrafish to five concentrations of the ayahuasca infusion: 0 (control), 0.1, 0.5, 1, and 3 ml/L ( = 14 each group), and behavior was recorded for 60 min. We evaluated swimming speed, distance traveled, freezing and bottom dwelling every min for 60 min. Swimming speed and distance traveled decreased with an increase in ayahuasca concentration while freezing increased with 1 and 3 ml/L. Bottom dwelling increased with 1 and 3 ml/L, but declined with 0.1 ml/L. Our data suggest that small amounts of ayahuasca do not affect locomotion and reduce anxiety-like behavior in zebrafish, while increased doses of the drug lead to crescent anxiogenic effects. We conclude that the temporal analysis of zebrafish behavior is a sensitive method for the study of ayahuasca-induced functional changes in the vertebrate brain.
茎和叶的混合浸液，即所谓的死藤水，几个世纪以来一直被土著部落使用。这种浸液富含N，N-二甲基色胺（DMT）和单胺氧化酶抑制剂，其特性与血清素相似。尽管在开发治疗焦虑和抑郁的新药方面取得了重大进展，但目前的治疗方法仍有几个局限性。像死藤水这样的替代药物可能会为这些疾病带来新的启示。在此，我们展示了死藤水在斑马鱼中引起的时间进程行为变化，这是朝着确定临床前评估的理想浓度迈出的第一步。我们将成年斑马鱼暴露于五种浓度的死藤水浸液中：0（对照）、0.1、0.5、1和3毫升/升（每组n = 14），并记录60分钟的行为。我们在60分钟内每分钟评估游泳速度、游动距离、僵住和底栖行为。游泳速度和游动距离随着死藤水浓度的增加而降低，而僵住在1和3毫升/升时增加。底栖行为在1和3毫升/升时增加，但在0.1毫升/升时下降。我们的数据表明，少量的死藤水不会影响运动，并能减少斑马鱼的焦虑样行为，而增加药物剂量会导致越来越明显的致焦虑作用。我们得出结论，斑马鱼行为的时间分析是研究死藤水诱导的脊椎动物大脑功能变化的一种敏感方法。
