Fritsma George A, McGlasson David L
The Fritsma Factor, Your Interactive Hemostasis Resource, 153 Redwood Drive, Trussville, AL, 35173, USA.
Clinical Research Scientist, San Antonio, TX, USA.
Methods Mol Biol. 2017;1646:333-347. doi: 10.1007/978-1-4939-7196-1_26.
Light transmittance aggregometry is the historical reference method for platelet function testing and continues to be used extensively. Whole blood impedance lumiaggregometry represents an updated methodology that provides for simplified specimen management, an assay milieu that replicates in vivo platelet activation conditions, improved reproducibility, and near-patient testing. While the impedance-based whole blood aggregometer with luminescence channel is becoming the standard for platelet function testing using this methodology, at least three near-patient whole blood instruments are available, each employing its unique technology. We provide descriptions of whole blood lumiaggregometry and three near-patient systems. We include the principle of operation, materials, and stepwise example protocols and speculate on the importance of concordance among the platforms.
透光率凝集测定法是血小板功能检测的历史参考方法，目前仍被广泛使用。全血阻抗光散射凝集测定法是一种更新的方法，它简化了样本管理，提供了模拟体内血小板激活条件的检测环境，提高了重现性，并可进行床旁检测。虽然带有发光通道的基于阻抗的全血凝集仪正成为使用该方法进行血小板功能检测的标准，但至少有三种床旁全血检测仪器可供使用，每种仪器都采用了独特的技术。我们对全血光散射凝集测定法和三种床旁检测系统进行了描述。我们介绍了操作原理、材料和逐步示例方案，并推测了各平台间一致性的重要性。
