Department of Biological Sciences, Oakland University, Rochester Hills, Michigan.
Rochester Adams High School, Rochester Hills, Michigan.
Curr Protoc. 2024 Jul;4(7):e1095. doi: 10.1002/cpz1.1095.
Aggregometry plays a crucial role in both clinical diagnostics and research within hematology, serving as a fundamental tool for understanding platelet function and its implications in physiological and pathological processes. In research, aggregometry provides insights into platelet aggregation dynamics and aids in understanding the underlying mechanisms of hemostasis, thrombosis, and related disorders. Light transmission aggregometry (LTA) and lumi-aggregometry, as well as whole blood aggregometry, are commonly employed methods. While LTA and lumi-aggregometry allow for specific platelet function assessment under controlled conditions, whole blood aggregometry provides a more physiologically relevant approach by evaluating platelet aggregation within the context of whole blood. Although both methodologies offer unique advantages, whole blood aggregometry allows for preservation of the native cellular environment, simplicity, and potential for better clinical correlation. In a clinical setting, with human blood samples, protocols are established for both LTA and whole blood aggregometry as they are frequently used diagnostic tools. A protocol for LTA and lumi-aggregometry in murine models has been described; however, to date, there is no standardized protocol for whole blood aggregometry in murine models accessible to hematology researchers. This article aims to outline a simple, basic protocol for murine whole blood aggregometry, offering an alternative method to the commonly used LTA aggregometry in research settings. Standardizing whole blood aggregometry protocols in murine models could enhance experimental reliability and facilitate translational research efforts in hematology. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Whole blood aggregometry in mice Support Protocol: Phenylhydrazine-induced anemia in wild-type mice Basic Protocol 2: Hematocrit percentage in mice.
聚集仪在血液学的临床诊断和研究中都起着至关重要的作用，是了解血小板功能及其在生理和病理过程中意义的基本工具。在研究中，聚集仪可以深入了解血小板聚集动力学，并有助于理解止血、血栓形成和相关疾病的潜在机制。常用的方法有光传输聚集仪（LTA）和发光聚集仪以及全血聚集仪。虽然 LTA 和发光聚集仪可以在受控条件下进行特定的血小板功能评估，但全血聚集仪通过评估全血中的血小板聚集提供了更具生理相关性的方法。虽然这两种方法都有其独特的优势，但全血聚集仪可以保留天然的细胞环境、操作简单，并可能与临床相关性更好。在临床环境中，使用人类血液样本，常为 LTA 和全血聚集仪建立方案，因为它们是常用的诊断工具。已经描述了用于鼠模型的 LTA 和发光聚集仪的方案；然而，迄今为止，在鼠模型中没有可用于血液学研究人员的标准化全血聚集仪方案。本文旨在概述一种简单的鼠全血聚集仪基本方案，为研究中常用的 LTA 聚集仪提供替代方法。在鼠模型中标准化全血聚集仪方案可以提高实验可靠性，并促进血液学的转化研究工作。
