Department of Polymer, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Tissue Repair Laboratory, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
J Biomed Mater Res B Appl Biomater. 2018 May;106(4):1595-1604. doi: 10.1002/jbm.b.33968. Epub 2017 Aug 14.
The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively. The proliferation and viability of Schwann cells onto the surfaces of SF/PEO nanofibrous scaffolds were investigated using SEM and thiazolyl blue (MTT) assay. The results showed an improvement of SF conformation and surface hydrophilicity of SF/PEO nanofibers after methanol and O plasma treatments. The immunostaining observation indicated a continuous coating of LN on the scaffolds. Improving the surface hydrophilicity and LN functionalization significantly increased the cell proliferation and this was more prominent after 5 days of culture time. In conclusion, the obtained results revealed that the electrospun LN-functionalized SF/PEO nanofibrous scaffold could be a promising candidate for peripheral nerve tissue regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1595-1604, 2018.
周围神经再生仍然是一个主要的临床问题，受到了极大的关注。在这项研究中，制备了电纺丝素蛋白（SF）/聚氧化乙烯（PEO）纳米纤维支架，并在其表面功能化层粘连蛋白（LN），而无需使用化学连接剂，以便潜在地用于周围神经组织工程。通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱、差示扫描量热法（DSC）和水接触角（WCA）测量分别对支架的形态、表面化学、热行为和润湿性进行了评估。使用 SEM 和噻唑蓝（MTT）测定法研究了雪旺细胞在 SF/PEO 纳米纤维支架表面的增殖和活力。结果表明，甲醇和 O 等离子体处理后，SF/PEO 纳米纤维的 SF 构象和表面亲水性得到改善。免疫染色观察表明 LN 连续涂覆在支架上。表面亲水性和 LN 功能化的提高显著增加了细胞增殖，在培养 5 天后更为明显。总之，研究结果表明，电纺 LN 功能化 SF/PEO 纳米纤维支架可能是周围神经组织再生的一种有前途的候选材料。
