对Linn. 抗氧化应激诱导的DNA和细胞膜损伤的评估。

Evaluation of Linn. against Oxidative Stress-induced DNA and Cell Membrane Damage.

作者信息

Kumar R Sunil, Narasingappa Ramesh Balenahalli, Joshi Chandrashekar G, Girish Talakatta K, Prasada Rao Ummiti Js, Danagoudar Ananda

机构信息

Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Hassan, Bengaluru, Karnataka, India.

Department of Studies and Research in Biochemistry, P.G Centre, Mangalore University, Chikka Aluvara, Kodagu, Karnataka, India.

出版信息

J Pharm Bioallied Sci. 2017 Jan-Mar;9(1):33-43. doi: 10.4103/0975-7406.206215.

DOI:10.4103/0975-7406.206215

PMID:28584491

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5450468/

Abstract

OBJECTIVE

The present study aims to evaluate antioxidants and protective role of Linn. against oxidative stress-induced DNA and cell membrane damage.

MATERIALS AND METHODS

The total and profiles of flavonoids were identified and quantified through reversed-phase high-performance liquid chromatography. antioxidant activity was determined using standard antioxidant assays. The protective role of extracts against oxidative stress-induced DNA and cell membrane damage was examined by electrophoretic and scanning electron microscopic studies, respectively.

RESULTS

The total flavonoid content of CtEA was 106.8 ± 2.8 mg/g d.w.QE, CtME was 72.4 ± 1.12 mg/g d.w.QE, and CtWE was 30.4 ± 0.8 mg/g d.w.QE. The concentration of flavonoids present in CtEA in decreasing order: quercetin >kaempferol >epicatechin; in CtME: quercetin >rutin >kaempferol; whereas, in CtWE: quercetin >rutin >kaempferol. The CtEA inhibited free radical-induced red blood cell hemolysis and cell membrane morphology better than CtME as confirmed by a scanning electron micrograph. CtEA also showed better protection than CtME and CtWE against free radical-induced DNA damage as confirmed by electrophoresis.

CONCLUSION

contains flavonoids and inhibits oxidative stress and can be used for many health benefits and pharmacotherapy.

摘要

目的

本研究旨在评估[植物名称未给出] Linn. 的抗氧化剂及其对氧化应激诱导的DNA和细胞膜损伤的保护作用。

材料与方法

通过反相高效液相色谱法鉴定并定量黄酮类化合物的总量和谱图。使用标准抗氧化剂测定法测定抗氧化活性。分别通过电泳和扫描电子显微镜研究检测[提取物名称未给出]提取物对氧化应激诱导的DNA和细胞膜损伤的保护作用。

结果

CtEA的总黄酮含量为106.8±2.8 mg/g干重QE，CtME为72.4±1.12 mg/g干重QE，CtWE为30.4±0.8 mg/g干重QE。CtEA中存在的黄酮类化合物浓度由高到低依次为：槲皮素>山奈酚>表儿茶素；CtME中为：槲皮素>芦丁>山奈酚；而CtWE中为：槲皮素>芦丁>山奈酚。扫描电子显微镜证实，CtEA比CtME更能抑制自由基诱导的红细胞溶血和细胞膜形态变化。电泳证实，CtEA对自由基诱导的DNA损伤的保护作用也优于CtME和CtWE。