Pramanik Sumit, Hanif Asyikin Sasha Mohd, Pingguan-Murphy Belinda, Abu Osman Noor Azuan
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia.
Materials (Basel). 2012 Dec 21;6(1):65-75. doi: 10.3390/ma6010065.
In this work, untreated bovine cortical bones (BCBs) were exposed to a range of heat treatments in order to determine at which temperature the apatite develops an optimum morphology comprising porous nano hydroxyapatite (nanoHAp) crystals. Rectangular specimens (10 mm × 10 mm × 3-5 mm) of BCB were prepared, being excised in normal to longitudinal and transverse directions. Specimens were sintered at up to 900 °C under ambient pressure in order to produce apatites by two steps sintering. The samples were characterized by thermogravimetric analysis, X-ray diffraction (XRD), and scanning electron microscopy (SEM) attached to an energy-dispersive X-ray spectroscopy detector. For the first time, morphology of the HAp particles was predicted by XRD, and it was verified by SEM. The results show that an equiaxed polycrystalline HAp particle with uniform porosity was produced at 900 °C. It indicates that a porous nanoHAp achieved by sintering at 900 °C can be an ideal candidate as an scaffold for load-bearing tissue applications.
在这项工作中，未处理的牛皮质骨（BCB）接受了一系列热处理，以确定磷灰石在哪个温度下会形成包含多孔纳米羟基磷灰石（nanoHAp）晶体的最佳形态。制备了尺寸为10 mm×10 mm×3 - 5 mm的BCB矩形试样，分别沿纵向和横向垂直切割。试样在常压下于高达900°C的温度下进行烧结，通过两步烧结法制备磷灰石。采用热重分析、X射线衍射（XRD）以及配备能量色散X射线光谱探测器的扫描电子显微镜（SEM）对样品进行表征。首次通过XRD预测了HAp颗粒的形态，并通过SEM进行了验证。结果表明，在900°C下生成了具有均匀孔隙率的等轴多晶HAp颗粒。这表明在900°C烧结获得的多孔nanoHAp可作为承重组织应用支架的理想候选材料。
