De Carvalho Bruno, Rompen Eric, Lecloux Geoffrey, Schupbach Peter, Dory Emilie, Art Jean-François, Lambert France
Department of Periodontology and Oral Surgery, University of Liège, 4000 Liège, Belgium.
Adjunct Professor, Augusta University, Augusta, GA 1120, USA.
Materials (Basel). 2019 Nov 28;12(23):3946. doi: 10.3390/ma12233946.
The influence of the manufacturing process on physicochemical properties and biological performance of xenogenic biomaterials has been extensively studied, but its quantification on bone-to-material contact remains poorly investigated. The aim of this study was to investigate the effect of different heat treatments of an experimental chemically-deproteinized bovine hydroxyapatite in vivo in terms of new bone formation and osteoconductivity. Protein-free hydroxyapatite from bovine origin was produced under sub-critical conditions and then either sintered at 820 °C or 1200 °C. Structural and morphological properties were assessed by scanning electron microscopy (SEM), measurement of surface area and X-ray diffractometry (XRD). The materials were then implanted in standardized alveolar bone defects in minipigs and histomorphometric evaluations were performed using non-decalcified sections. Marked topographical differences were observed by SEM analysis. As the sintering temperature of the experimental material increased, the surface area significantly decreased while crystallite size increased. In vivo samples showed that the highly sintered BHA presented a significantly lower percentage of newly formed bone than the unheated one ( = 0.009). In addition, the percentage of bone-to-material contact (BMC) was significantly lowered in the highly sintered group when compared to the unsintered ( = 0.01) and 820 °C sintered ( = 0.02) groups. Non-sintered or sintered at 820 °C BHA seems to maintain a certain surface roughness allowing better bone regeneration and BMC. On the contrary, sintering of BHA at 1200 °C has an effect on its morphological and structural characteristics and significantly modify its biological performance (osteoconductivity) and crystallinity.
制造工艺对异种生物材料的物理化学性质和生物学性能的影响已得到广泛研究，但其对骨与材料接触的量化研究仍很少。本研究的目的是在体内研究实验性化学脱蛋白牛羟基磷灰石不同热处理对新骨形成和骨传导性的影响。在亚临界条件下制备牛源无蛋白羟基磷灰石，然后在820℃或1200℃烧结。通过扫描电子显微镜（SEM）、表面积测量和X射线衍射（XRD）评估结构和形态特性。然后将材料植入小型猪的标准化牙槽骨缺损中，并使用非脱钙切片进行组织形态计量学评估。通过SEM分析观察到明显的形貌差异。随着实验材料烧结温度的升高，表面积显著减小而微晶尺寸增大。体内样本显示，高度烧结的BHA新形成骨的百分比明显低于未加热的BHA（P = 0.009）。此外，与未烧结组（P = 0.01）和820℃烧结组（P = 0.02）相比，高度烧结组的骨与材料接触（BMC）百分比显著降低。未烧结或820℃烧结的BHA似乎保持一定的表面粗糙度，有利于更好的骨再生和BMC。相反，1200℃烧结的BHA对其形态和结构特征有影响，并显著改变其生物学性能（骨传导性）和结晶度。
