Nowacka-Woszuk Joanna, Madeja Zofia E, Chmurzynska Agata
Department of Genetics and Animal Breeding, Poznań University of Life Sciences, Wolynska 33, 60-637, Poznan, Poland.
Department of Human Nutrition and Hygiene, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624, Poznan, Poland.
BMC Genet. 2017 Aug 15;18(1):78. doi: 10.1186/s12863-017-0544-0.
Undernutrition is an increasingly common problem. Insufficient calorie intake and nutrient deficiencies during pregnancy may have an impact not only on the mother, but may also alter metabolism in the infant. In this study, we have applied a calorie-restricted diet during gestation and examined its effect on hepatic Fasn mRNA and DNA methylation profiles in rats and their female progeny. The body composition and blood lipid profiles were also evaluated in both generations.
The results showed that the investigated diet regimen exerted a greater effect on the dams than on the offspring. We found that, in the calorie-restricted group, the transcript level of the Fasn gene in the liver increased in the mothers, while in the progeny it was only slightly enhanced. The implemented diet altered lipid profile in the dams by decreasing total cholesterol, HDL, and TG levels. An increase in LDL was noted in the offspring. No change in DNA methylation profile was observed in response to the calorie-restricted diet.
Calorie restriction during pregnancy modified the hepatic Fasn mRNA transcript level and altered the blood cholesterol concentrations in dams, but there were no such effects in their four-week-old offspring. The examined dietary regimen had no effect on DNA methylation of the Fasn 5'-flanking region in the rat liver.
营养不良是一个日益常见的问题。孕期热量摄入不足和营养缺乏不仅可能影响母亲，还可能改变婴儿的新陈代谢。在本研究中，我们在孕期应用了热量限制饮食，并研究了其对大鼠及其雌性后代肝脏脂肪酸合酶（Fasn）mRNA和DNA甲基化谱的影响。同时还评估了两代动物的身体组成和血脂谱。
结果表明，所研究的饮食方案对母鼠的影响大于对后代的影响。我们发现，在热量限制组中，母亲肝脏中Fasn基因的转录水平升高，而后代中仅略有增强。所实施的饮食通过降低总胆固醇、高密度脂蛋白和甘油三酯水平改变了母鼠的血脂谱。后代中低密度脂蛋白有所增加。未观察到热量限制饮食对DNA甲基化谱有影响。
孕期热量限制改变了母鼠肝脏中Fasn mRNA转录水平并改变了其血液胆固醇浓度，但对其四周龄后代没有此类影响。所研究的饮食方案对大鼠肝脏中Fasn 5'侧翼区域的DNA甲基化没有影响。
