He Tao, Zhang Haijun, Wang Jing, Wu Shugeng, Yue Hongyuan, Qi Guanghai
Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2017 Aug 15;12(8):e0182886. doi: 10.1371/journal.pone.0182886. eCollection 2017.
Cottonseed meal (CSM) is commonly used in hens' diets to replace soybean meal (SBM). However, the molecular consequences of this substitution remains unclear. To investigate the impact of this substitution at the molecular level, iTRAQ combined with biochemical analysis was performed in Hy-Line W-36 hens supplemented with a mixed diet of CSM and SBM. Egg weight, albumen height, and Haugh unit were significantly reduced in the CSM100 group (100% crude protein of SBM replaced by CSM) compared with the SBM group (P<0.05). A total of 15 proteins, accounting for 75% of egg white proteins with various biological functions of egg whites, were found to be reduced. This finding may relate to the decrease of albumen quality in the CSM100 group. Oviduct magnum morphology and hormone analysis indicated that a reduced level of plasma progesterone caused reduced growth of the tubular gland and epithelial cells in the magnum, further decreasing egg white protein synthesis in the magnum. These findings help demonstrate the molecular mechanisms of a CSM diet that cause adverse effects on albumen quality, while also showing that SBM should not be totally replaced with CSM in a hen diet.
棉籽粕（CSM）常用于母鸡日粮中以替代豆粕（SBM）。然而，这种替代的分子后果仍不清楚。为了在分子水平上研究这种替代的影响，对饲喂CSM和SBM混合日粮的海兰W-36母鸡进行了iTRAQ结合生化分析。与SBM组相比，CSM100组（用CSM替代100%豆粕粗蛋白）的蛋重、蛋白高度和哈夫单位显著降低（P<0.05）。共发现15种蛋白质减少，占具有蛋清各种生物学功能的蛋清蛋白质的75%。这一发现可能与CSM100组蛋清质量下降有关。输卵管膨大部形态和激素分析表明，血浆孕酮水平降低导致膨大部管状腺和上皮细胞生长减少，进而减少膨大部蛋清蛋白质合成。这些发现有助于阐明CSM日粮对蛋清质量产生不利影响的分子机制，同时也表明在母鸡日粮中不应完全用CSM替代SBM。
